摘要
以啤酒酵母（Saccharomycescerevisiae）菌株Ⅰ为出发株，经紫外线处理后，在含可完全抑制出发株生长的乙硫氨酸（浓度为2g/L）的基本培养从上筛出14株生长良好的突变株，其中I－2，I－9株还能在无硫基本培养基上良好生长。经氨基酸自动分析仪检测，I－2株菌体蛋白中的蛋氨酸含量（质量由分比）由I株的1．93增年9．28。
The cells of Saccharomyces cerevisiae strain I was incubated on mineral medium (MM)which conained 2. 0g/L of ethionine after treated with ultraviolet. The concentration ofethionine inhibits the growth of the strain. Fourteen well - growing colonies was screenedfrom the basic medium where could totally inhibit the growth of the starting strain. Reason-ably, they are ethionine resistant mutants. Two of the mutants I - 2 and I - 9 also grew wellin MM lacking sulfur, and I - 2 was confirmed as methionine high - yield strain with auto-matic amino acid analyzer to analyze its amino acid to the whole cell protein. The content ofmethionine increased fon 1. 93 (w/w, % ) of strain I to 9. 28 (w/w, % ).
出处
《微生物学杂志》
CAS
CSCD
1999年第1期6-8,共3页
Journal of Microbiology
关键词
蛋氨酸
乙硫氨酸
高产酵母突变株
筛选
methionine
ethionine
structural analogue
growth in medium lacking sulfur
