摘要
目的：研究脑缺血后一氧化氮合酶（ＮｉｔｒｉｃＯｘｉｄｅＳｙｎｔｈａｓｅ，ＮＯＳ）表达与细胞凋亡的关系。方法：采用大鼠右侧大脑中动脉闭塞（Ｍｉｄｄｌｅｃｅｒｅｂｒａｌａｒｔｅｒｙｏｃｃｌｕｓｉｏｎ，ＭＣＡＯ）模型，观察脑梗塞体积、ＮＯＳ表达、细胞凋亡及ＮＯＳ抑制剂硝基－Ｌ－精氨酸（Ｌ－ＮＡ）、ＮＯ供体硝普钠（ＳＮＰ）对上述结果的影响。结果：ＮＯＳ表达与缺血再灌流（ＩＲ）早期细胞凋亡有关，ＳＮＰ增加ＩＲ早期细胞凋亡，Ｌ－ＮＡ能够抑制ＩＲ早期的细胞凋亡，但使脑梗塞体积增大。结论：ＩＲ早期ＮＯＳ表达对细胞凋亡有促进作用。
Objective: To study the relationship between the quantity of nitric oxide (in term of the expression of NO synthase) and neuronal apoptosis after focal cerebral ischemia. Methods: Focal cerebral ischemia was induced in adult wistar rats by ligating the right middle cerebral artery. The area of brain infarct was measured with TTC staining. NO synthase (NOS) was determined with NADPHd histochemistry. Neuronal apoptosis was measured with TUNEL technique and the proportion of apoptotic neurons with FCM. Results: The number of NADPHd positive cells was positively correlated to the number of apoptotic neurons in the early stage. SNP, a donor of NO, increased not only the number of NOS expressing cells but also the proportion of TUNEL positive cells 30 minutes to 6 hours after reperfusion. L NA, an inhibitor of NOS, decreased the number of NOS expressing cells and the apoptotic neurons but enlarged the area of infarct. Conclusion: NO may be involved to neuronal apoptosis in the early stage after the onset of focal cerebral ischemia.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1999年第4期249-252,共4页
Journal of Third Military Medical University
基金
国家自然科学基金
关键词
一氧化氮合酶
脑缺血
细胞凋亡
大鼠
nitric oxide synthase
cerebral ischemia
apoptosis
rat
