摘要
目的：探讨趋化性细胞因子巨噬细胞炎症蛋白－２（ＭＩＰ－２）在内毒素诱导急性肺损伤（ＡＬＩ）中的可能作用。方法：采用斑点杂交及原位杂交技术对ＡＬＩ大鼠肺组织中ＭＩＰ－２ｍＲＮＡ的表达进行定量和定位研究。结果：内毒素性ＡＬＩ各组大鼠肺组织ＭＩＰ－２ｍＲＮＡ表达量显著高于对照组，且在峰值水平与血浆及肺组织匀浆髓过氧化物酶（ＭＰＯ）活力呈正相关（ｒ＝０．７２９，ｒ＝０．８８５，Ｐ＜０．０５）。原位杂交发现在ＡＬＩ早期肺内巨噬细胞是ＭＩＰ－２的主要分泌细胞。结论：肺巨噬细胞释放的ＭＩＰ－２可能是ＡＬＩ时多形核白细胞（ＰＭＮ）在肺内大量浸润并激活的原因之一，因而参与了ＡＬＩ的早期发病过程。
Objective: To study the potential role of chemotactic macrophage inflammatory protein 2 (MIP
2) in the development of LPS induced acute lung injury (ALI). Methods: The amount and
distribution of MIP 2 were determined in the lungs with ALI. Results: The expression of MIP 2
mRNA was significantly higher in the lungs of the animals with ALI than those of the control ( P
<0.01). In addition, The expression level of MIP 2 mRNA was positively correlated to the
activity of myeloperoxidase in the plasma and lung homogenate ( r =0.729, r =0.885 and P
<0.05). It was confirmed with in situ hybridization that pulmonary macrophages were the
predominant source of MIP 2 in the early stage of ALI. Conclusion: MIP 2 released mainly from
pulmonary macrophages might contribute to the activation and sequestration of
polymorphonuclear leucocytes in the lungs challenged with LPS. So MIP 2 might take part in
the development of LPS induced ALI.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1999年第4期235-237,共3页
Journal of Third Military Medical University
基金
国家自然科学基金
