摘要
报告一种大鼠活体多次采血法。用1%戊巴比妥钠腹腔麻醉后,剑突下进针,斜行向上刺入2-5~3.0cm即达心脏,回抽。30只SD大鼠,间隔10 d采血2 ml,共4次,测定Hb、RBC、Hct、WBC、ALT、TP、BUN。结果:采血1000余次,1次穿刺成功率98%以上,且大鼠成活,各次血液成份及生化指标差异均无显著性(P>0-05)。本法操作简便,定位准确,采血量大,可重复采血。
We have established a new method which can gather more blood repeatedly in living rats.The rats were anaesthetized by intraperitoneal injection with 1%sodium pentobarbital,then penetrated upward obliquely at the site of just below the xiphoid,entered about 2.5~3.0 cm into the heart and drew blood out.Two ml blood has been extracted from 30 SD rats every 10 days 4 times in all,and the Hb,RBC,Hct,WBC,ALT,TP and BUN have been determined.Results:by using this method,the rate of one time successful penetration is over 98%without sacrificing animals,and no significant difference(P>0.05)has been detected in blood components and biochemical targets each time.The method is easy to control and is applicable for gathering more blood repeatedly in living rats.
作者
盛晖
Sheng Hui(Department of General Surgery,First Affiliated Hospital,Hubei Mextical University,Wuhan 430060,China)
