摘要
用谷胱甘肽琼脂糖凝胶亲和层析法从中国广西水牛源大片吸虫（Ｆａｓｃｉｏｌａｇｉｇａｎｔｉｃａ）成虫体内提纯大片吸虫谷胱甘肽Ｓ－转移酶（ＦｇＧＳＴ）。提纯的ＦｇＧＳＴ在１２％ＳＤＳ－ＰＡＧＥ图谱上呈现二条清晰谱带，分子量分别为２６．５ｋＤ和２８ｋＤ。以ＣＤＮＢ为标准底物测得ＦｇＧＳＴ的活力为１７．０８μｍｏｌ／ｍｉｎｍｇ。用谷胱甘肽亲和层析柱纯化的ＦｇＧＳＴ，不仅纯度好，而且获得率高，所得ＦｇＧＳＴ至少占上柱上清液总蛋白含量的３．１％。
In our experiment, Fasciola gigantica glutathione s transferase(FgGST) was isolated from crude extracts of Fasciola gigantica by glutathione agrose affinity chromatapaphy. SDS PAGE shows two closely migrating polypeptides with molecular weight of 26 5 kD and 28 kD respectively. The substance has GST activity of 17 08 μmol/min·mg, when measured using CDNB as standard substrate. And Fasciola gigantica has high concentration of GST. For each isolation 100 mg protein of F g extract is loaded onto the affinity column and the yield of FgGST obtained is at least 3 1% of the total protein.
出处
《广西科学》
CAS
1999年第1期69-71,共3页
Guangxi Sciences
基金
国家自然科学基金
关键词
大片吸虫
提纯
生化分析
水牛
GST
吸虫
Glutathione S Transferase, Fasciola gigantica ,isolation,biochemical characterisation,water buffalo
