摘要
目的:探讨维生素A(VitA)和锌(Zn)摄入过量对幼龄大鼠脾脏抗氧化能力的影响,并为VitA和Zn的营养补充上限的制定提供实验室参考依据。方法:通过灌胃造成VitA摄入10倍于推荐摄入量(RNI)的大鼠动物模型,摄入剂量为0.48g/kg,Zn摄入20倍于RNI的大鼠动物模型,摄入剂量为1.00g/kg,并按相同剂量设立联合过量组,同时设立对照组。在实验第4周末处死全部大鼠,制备脾脏组织匀浆,测定大鼠脾脏总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量。应用SPSS15.0统计软件对其测定结果进行统计分析。结果:实验组脾脏中T-AOC、SOD、GSH-Px、CAT活性明显降低,MDA含量显著升高,与对照组相比差异有统计学意义(P<0.05),而且VitA和Zn联合过量组较之VitA和Zn单一过量组差异更明显。结论:过量补充VitA和Zn可降低幼龄大鼠脾脏的抗氧化能力。
Objective：To investigate the effect of excessive intake of vitamin A and Zinc （Zn） on the antioxidant capacity of young rats＇ spleen,and to provide the laboratory reference of the upper limit of the vitamin A and Zinc＇s nutritional supplement.Methods：Instill 0.48 g/kg dose VA into the rats＇stomach resulted one animal model which 10 times VA＇s RNI.And 1.00 g/kg dose Zn resulted another animal model which 20 times Zn＇s RNI.According to the same dose,a joint excessive group of VA and Zn was established.At the same time a contol group was established.Kill all the rats at the end of 4th weekend of the experiment.And the spleen tissue homogenate was prepared,the rats＇ spleen total antioxidant capacity （T-AOC）,superoxide dismutase （SOD）,glutathione peroxidase （GSH-Px）,catalase （CAT） and malondialdehyde vitality （MDA） were determined.The statistic results were analyzed with SPSS15.0 software.Results：The spleen＇ s T-AOC,SOD,GSH-Px,CAT activity decreased significantly,and MDA was significantly increased in experiment group.Compared with the contol group,there were significantly difference （P 0.05）.And the differents were more significant between the joint excessive group of VA and Zn and the separate excessive group.Conclusion：The excessive supplementary of VA and Zn can reduce the antioxidant capacity of the rats＇ spleen.
出处
《中国妇幼保健》
CAS
北大核心
2010年第28期4136-4138,共3页
Maternal and Child Health Care of China
关键词
维生素A
锌
脾脏
抗氧化能力
Vitamin A （VA）
Zinc （Zn）
Spleen
Antioxidant capacity
