摘要
为研制能够同时预防传染性法氏囊病(IBD)和新城疫(ND)的疫苗,本研究选用表达IBD病毒(IBDV)超强毒株(vvIBDV)VP2基因的重组ND病毒(NDV)LaSota疫苗株(rL-VP2),测定其半数鸡胚感染量、平均鸡胚致死时间、脑内接种指数和静脉内致病指数等指标,按不同剂量接种18胚龄SPF鸡胚,分别于出雏后第9d、第14d和第21d采血,用微量凝集法和ELISA方法测定血清中抗NDV抗体和抗IBDV抗体水平,并于出雏后28d用NDV强毒(F48E9株)和vvIBDVGx株攻毒,评估重组疫苗的胚胎免疫效果。结果显示:按104EID50/枚剂量进行免疫不会影响SPF鸡胚出雏率和出雏后21d存活率,并且该免疫组雏鸡能够对NDV强毒和vvIBDV攻毒提供安全的免疫保护。
The recombinant Newcastle disease virus （NDV） （rL-VP2） expressing the VP2 gene of very virulent infectious bursal disease virus （vvIBDV） Gx strain as a candidate vaccine was evaluated. The vaccine was administered to 18 day old SPF chicken embryos with different doses and antibody titers were detected at 9,14 and 21 days post hatching. The results showed that the vaccine did not hamper hatching or surviving post hatching when rL-VP2 was administered with 104 EID50/egg dosage. The rL-VP2 vaccination could completely protect chickens against the NDV F48E9 strain and vvIBDV Gx strain challenge at four weeks post-hatch.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2010年第9期695-698,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
国家科技支撑计划(2006BAD06A16)
国家科技支撑计划(2006BAD06A05)
关键词
胚胎免疫
重组新城疫病毒
传染性法氏囊病毒超强毒株
VP2基因
in ovo vaccination
recombinant Newcastle disease virus
very virulent Infectious bursal disease virus
VP2 gene
