摘要
采用MTT法测定不同浓度的OPS单独或协同伴刀豆球蛋白(ConA)对鸡淋巴细胞增殖能力的影响。结果表明:单独刺激外周血淋巴细胞时,OPS在0.0781~20 mg·mL^-1时,其吸光度值均显著高于细胞对照组;协同ConA刺激外周血淋巴细胞时,OPS+ConA在0.3125~20 mg·mL^-1时,其吸光度值均显著高于ConA对照组。单独刺激脾脏淋巴细胞时,OPS在0.3125~20 mg·mL^-1时,其吸光度值均显著高于细胞对照组;协同ConA刺激脾脏淋巴细胞时,OPS+ConA在0.3125~20 mg·mL^-1时,其吸光度值均显著高于ConA对照组。由此表明OPS在体外既可以单独刺激鸡外周血淋巴细胞和脾脏淋巴细胞增殖,又与ConA具有协同刺激作用,且在一定浓度范围内,呈现出明显的剂量反应关系。
MTT method was used to determine the effect of OPS alone or in combination with concanavalin(ConA)on the proliferation of chicken lymphocytes.When OPS were 0.0781~20 mg·mL^-1,its absorbance value was significantly higher than that of the cell control group(P<0.05);when OPS+ConA were 0.3125~20 mg·mL^-1,its absorbance value was significantly higher than that of the con a control group(P<0.05).When OPS were 0.3125~20 mg·mL^-1 alone,the absorbance value of OPS was significantly higher than that of cell control group(P<0.05);when OPS+ConA were 0.3125~20 mg·mL^-1,the absorbance value of OPS+ConA were significantly higher than that of ConA control group(P<0.05).It is suggested that OPS can not only stimulate the proliferation of peripheral blood lymphocytes and spleen lymphocytes in vitro,but also have synergistic effect with ConA,and in a certain concentration range,it has a significant dose-response relationship.
作者
夏继涛
成好学
姚宏亮
张萍
XIA Ji-tao;CHENG Hao-xue;YAO Hong-liang;ZHANG Ping(Shanghai Ocean University, Shanghai 201306, China;Jinling Institute of Technology, Nanjing 210038, China)
出处
《金陵科技学院学报》
2020年第3期82-86,共5页
Journal of Jinling Institute of Technology
基金
金陵科技学院大学生创新训练计划项目(201913573084X)
江苏省农业科技自主创新资金项目(CX〔17〕3035)
金陵科技学院校级“创客”虚拟班持续建设项目(2017009,2017008)。
关键词
黄秋葵多糖
淋巴细胞
免疫增殖
okra polysaccharides
lymphocytes
immunoproliferation
