摘要
以珠美海棠为材料,研究了培养基配比、苗龄、琼脂等组培苗工厂化生产关键技术,光照条件和培养容器对组培苗生长发育的影响。结果表明,采用改良型培养基MS+6-BA0.5mg/L+IAA0.2mg/L用于分化培养、1/2MS+IAA0.5mg/L或1/2MS+IBA0.5mg/L用于生根培养,苗龄30～40d,使用接近国际标准的琼脂、光照强度1500～2000lx条件下生产的组培苗繁殖系数最大、有效苗最多、生根率高及生长速度最快,移栽成活率>90％;苗圃移栽成活率>85％,1年生苗株高均>1.5m。并建立了珠美海棠工厂化生产工艺及组培苗培养标准,为其他植物组培苗繁殖提供理论依据。
; Culturing the stems of Malus zumi in vitro,the key techniques including medium, auxin, age of sprouts and agar are studied. The results show that if there is the advanced medium MS + 6-BA 0. 5mg/L + IAAO. 2mg/L for multiplicating,1/2MS + IAA0. 5mg/L or 1/2MS+IBAO. 5mg/L for rooting,sprout's age being 30-40 days,agar corresponding to the international standard and the light intensity sustaining at 1500-2000lx,the coefficient of multiplication,the quantity of effictive plantlets and the rate of rootage and growth are best, and the surviving ratio of transplantation of plantlets and nursery seedling is above 90% and 85% respectively,and 1-yr-old tree height is above 1. 5m. Based on the study,the industrializing technology of tube-plantlets of Malus zumi and the culture standard which improves a theoretical basis for vegetive propagation of others plant are summarized.
出处
《生态农业研究》
CSCD
1999年第2期50-52,共3页
Chinese Journal of Eco-agriculture
基金
"八五"国家重点推广项目
关键词
珠美海棠
组培苗
繁殖
培养基
Malus zumi,Tissue-culture sprout ,Propagative technology,Culture medium
