摘要
目的研究植物乳杆菌ST-Ⅲ对大肠埃希菌和沙门菌与Caco-2细胞粘附的抑制作用,并初探其机制。方法采用CFDA-SE荧光标记的方法测定加入ST-Ⅲ前后对致病菌对Caco-2细胞粘附能力的变化,通过化学和酶处理ST-Ⅲ细胞壁表面成分、提取相关物质,研究ST-Ⅲ对致病菌粘附的抑制机制。结果 ST-Ⅲ对2种致病菌都具有明显的抑制粘附的能力,其在相同情况下对大肠埃希菌粘附的抑制效果好于对沙门菌的效果(P<0.05)。化学和酶处理表明ST-Ⅲ的表面蛋白或者磷壁酸可能参与了对致病菌粘附的抑制过程,提取后发现表面蛋白对大肠埃希菌的粘附表现出极强的抑制效果(P<0.01),而对沙门菌的粘附没有抑制作用(P>0.05);磷壁酸对2种致病菌均不具备抑制粘附的作用。结论 ST-Ⅲ对大肠埃希菌与Caco-2细胞粘附的抑制作用主要是通过竞争性抑制,而对沙门菌粘附的抑制作用主要是通过空间位阻形成的。
Objective To study the antagonistic activities of Lactobacillus plantarum ST-Ⅲ against the adhesion of Escherichia coli and Salmonella enteritis to Caco-2 cells and explicate the phenomena of bacterial adhesion and inhibition.Method Fluorescence labeling with CFDA-SE was used to study the change of adhesive ability to Caco-2 cells of the two pathogens;the mechanisms of antagonistic activities were investigated by chemical and enzymatic treatments and extraction of component on the cell surface.Result ST-Ⅲ showed significant capability to inhibit the adhesion of E.coli and S.enteritis to Caco-2 cells.ST-Ⅲ had a better antagonistic effects against E.coli than against S.enteritis in the same situations.Chemical and enzymatic treatments showed the S-layer protein or lipoteichoic acid might be involved in the adhesion of ST-Ⅲ to Caco-2 cells.After extracted,the S-layer protein showed significantly inhibitory efforts to the adhesion of E.coli,but could not inhibit the adhesion of S.enteritis.The lipoteichoic acid on ST-Ⅲ cells did not affect the adhesion of pathogens in all experiments.Conclusion The antagonistic effect of ST-Ⅲ against the adhesion of E.coli on Caco-2 cells was ascribed to competitive inhibition,whereas the adhesion of S.enteritis to Caco-2 cells was mainly resulted from steric hindrance.
出处
《中国微生态学杂志》
CAS
CSCD
2010年第9期773-777,780,共6页
Chinese Journal of Microecology
基金
国家"十一五"科技支撑计划项目(2006BAD04A14)
关键词
荧光标记法
植物乳杆菌
粘附抑制
Fluorescence labeling
Lactobacillus p/antarum
Inhibition of adhesion
