摘要
介绍一种眼组织透射电子显微镜标本制备方法。组织先用４％ 多聚甲醛—２．５％ 戊二醛混合固定液预固定，１％ 四氧化锇固定液后固定，丙酮逐级脱水，延长Ｅｐｏｎ８１２ 包埋液的浸透时间并包埋，用玻璃刀或钻石刀作超薄切片，醋酸铀及枸椽酸铅双重染色，Ｈ６００Ⅳ型透射电子显微镜观察。此方法的优点在于眼组织各层超微结构保存良好，没有损伤及变性，我们对该方法的操作要点作了讨论。
A method of preparing the section of eye tissue for transmission electron microscopy (TEM) is recommended. The tissue was prefixed with a mixed solution of 4% paraformaldehyde and 2.5% glutaraldehyde and followed by softening in 3% EDTA solution for 20 minutes, then the tissue was fixed in 1% osmium tetroxide, dehydrated in series acetone, infiltrated in Epox 812 for a longer, and embeded.Ultrathin sections were cut with glass knives or diamond knife, stained with uranyl acetate and lead citrate and examined with H600 Ⅳ. The advantage of this method is that the ultrastructures of tissues are well preserved without any damage and distortion. The main points of the procedure of preparation have been discussed.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
1999年第2期237-239,共3页
Journal of Biomedical Engineering
关键词
眼组织
透射电子显微镜
电镜样品
制备
Eye tissue Transmission electron microscopy Preparation of specimen for TEM
