摘要
目的：通过离体及整体实验观察了人和大鼠肠内菌对人参皂甙Ｒｂ１（ＧＲｂ１）的代谢。方法：采用薄层色谱（ＴＬＣ）和电喷雾质谱（ＥＳＩＭＳ）检测ＧＲｂ１及其代谢产物。结果：离体实验表明，ＧＲｂ１容易被大鼠和人消化道菌群代谢，随着代谢时间的延长，相继出现Ｒｄ，Ｒｇ３／Ｆ２，Ｒｈ２／ＣＫ和Ｐｐｄ４种代谢产物。给大鼠ｉｇＧＲｂ１５００ｍｇ·ｋｇ－１后收集４ｈ和６ｈ粪，提取ＧＲｂ１的代谢产物，证明粪中存在Ｒｄ和Ｒｇ３／Ｆ２两种代谢产物。结论：ＧＲｂ１可被人和大鼠肠内菌代谢，其代谢模式为ＧＲｂ１→Ｒｄ→Ｆ２→ｃｏｍｐｏｕｎｄＫ（ＣＫ）→２０（Ｓ）ｐｒｏｔｏｐａｎａｘａｄｉｏｌ（Ｐｐｄ）。
AIM: To study the metabolism of ginsenosid Rb1(GRb1) by rat and human intestinal bacteria in vitro and in vivo. METHODS: Using thinlayer chromatography(TLC) and electron spurt ion mass (ESIMS) for measurements of GRb1 and its metabolites. RESULTS: GRb1 was decomposed easily in vitro by rat and human intestinal bacteria, and consequently four metabolites (Rd, Rg3/F2, Rh2/CK and Ppd) were observed on TLC with prolongation of incubation time. Using electron spurt ion mass spectroscopy(ESIMS), GRb1 and its metabolites in rat feces were also assayed 4 h and 6 h after oral administration. Both metabolites Rd and Rg3/F2 were detected. CONCLUSION: The above mentioned results showed that the mode of metabolism of GRb1 was Rb1RdF2CK and the final metabolite was 20(S) Ppd in the feces of rats and human.
出处
《药学学报》
CAS
CSCD
北大核心
1999年第6期410-414,共5页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
关键词
人参皂甙RB1
肠内菌代谢
离体实验
TC
ESI-MS
ginsenoside Rb1
intestinal bacteria metabolism
electron spurt ion mass spectroscopy(ESIMS)
thinlayer chromatography(TLC)
