摘要
诱导体外培养扩增后的小鼠骨髓ＣＤ３４＋造血细胞分化为树突状细胞（ＤＣ）。方法Ｃ５７ＢＬ／６ｊ小鼠骨髓ＣＤ３４＋细胞采用血管内皮细胞共培养方法进行体外扩增，扩增后的ＣＤ３４＋细胞经磁性细胞分选技术纯化，并用ＧＭ┐ＣＳＦ等细胞因子诱导其向ＤＣ分化。结果小鼠骨髓ＣＤ３４＋造血细胞不论是否经过体外培养均可被诱导分化为ＤＣ，后者具有典型的树突状形态特征，表达高水平的ＭＨＣⅠ、Ⅱ类抗原和ＣＤ８６共刺激分子，在混合白细胞反应（ＭＬＲ）中能有效地刺激静息期Ｔ淋巴细胞增殖。结论小鼠骨髓ＣＤ３４＋细胞经体外培养扩增后仍然具有向ＤＣ分化的能力，通过骨髓细胞体外培养扩增可以显著提高ＤＣ产量。
Objective:To induce the dendritic cell (DC) differentiation from murine bone marrow CD34 + hematopoietic cells expanded ex vivo.Methods:The marrow CD34 + cell of C37BL/6j mice were expanded ex vivo by using a bone marrow microvessel endothelial cell co culture method.The expanded CD34 + cells were purified by MACS and then induced by cytokines including GM CSF to differentiate into DC.Results:The murine bone marrow CD34 + cells can be induced in vitro for DC differentiation,regardless of expanded ex vivo or not.The obtained cells had a typical morphology constant to DC,expressed a high level of MHC class Ⅰ and Ⅱ antigens as well as CD86 co stimulator molecule,and were potent stimulators for quiescent T lymphocytes in the mixed leukocyte reaction.Furthermore,the expanded marrow cells can be induced directly into DC without the need of purification procedure,and thereby the yield of DC can be further increased.Conclusion:Murine marrow CD34 + cells after expanded ex vivo remained the capacity of differentiating into DC.The yield of DC can be, therefore, increased significantly by ex vivo expansion of the marrow hematopoietic cells.
出处
《肿瘤》
CAS
CSCD
北大核心
1999年第3期132-136,共5页
Tumor
基金
国家自然科学基金
上海市卫生系统"百人计划"资助
