摘要
目的:研究枸杞多糖(Lyciumbarbarumpolysaccharide,LBP)对荷瘤小鼠体内肿瘤微环境中T淋巴细胞亚群和树突状细胞(dendriticcells,DCs)的影响,探讨LBP对荷瘤机体免疫逃逸的干预作用。方法:用LBP给H22荷瘤小鼠灌胃,连续2周后,测定肿瘤重量,采用流式细胞术检查肿瘤浸润淋巴细胞(tumor-infiltratinglymphocyte,T1L)中T淋巴细胞亚群和DCs的数量,以及协同刺激分子CD80(B7-1)表达的变化。结果:LBP可明显抑制肿瘤细胞的生长,增加肿瘤浸润CD4+、CD8+。细胞的数量。LBP高剂量组CD4+和CD8+细胞数量的百分比高于模型组(P<0.05)。LBP低剂量和高剂量组肿瘤浸润DCs数量及B7-1表达均较模型组升高,但差异无统计学意义。结论:LBP的抗肿瘤作用可能与恢复荷瘤小鼠TIL中CD4+、CD8+的细胞数量、解除机体的免疫抑制状态及增强机体的抗肿瘤免疫功能有关。LBP能否恢复荷瘤机体DCs的表型及功能尚待进一步研究。
Objective： To study the effects of Lycium barbarurn polysaccharide （LBP） on tumor microenvironment T-lymphocyte subsets and dendritic cells in H22-bearing mice and the mechanisms for intervention of tumor immune escape by LBP. Methods： H22-bearing mice were given LBP orally for two weeks. T-lymphocyte subsets and the phenotypes of dendritic cells in tumor-infiltrating lymphocytes （TIL） were detected by flow cytometry （FCM）. Results： LBP could significantly increase the numbers of CD4^＋ and CD8^＋ T cells in TIL as compared with those in model control group （P〈0.05）. In model control group, the number of dendritic cells in tumor microenvironment decreased markedly, while in LBP-treated group, the increased number of dendritic cells and B7-1 expression were observed, but there were no significant differences between these two groups. Conclusion： LBP has anti-tumor effect probably by increasing the numbers of CD4^＋ and CD8^＋ T cells in TIL to relieve the immunosuppression and enhance the anti-tumor function of the immune system. But whether LBP can recover the phenocyte and function of dendritic cells in H22-bearing mice should be further studied.
出处
《中西医结合学报》
CAS
2005年第5期374-377,共4页
Journal of Chinese Integrative Medicine
基金
广东省中医药管理局资助项目(No.101054)
关键词
枸杞多糖
免疫抑制
淋巴细胞亚群
肿瘤浸润淋巴细胞
树突状细胞
Lycium barbarum polysaccharide~ immunosuppression~ T-lymphocyte subsets
tumorinfiltrating lymphocyte~ dendritic cells
