摘要
目的：早期诊断关节结核。方法：应用聚合酶链反应（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＰＣＲ）技术对３３例共３８个关节的关节液行结核杆菌检测；同时将所取关节液抗酸染色涂片镜检，以病理诊断为金标准并比较其结果。结果：３８个关节经病理确诊的关节结核有１７个，其中ＰＣＲ法与涂片法检出的阳性数分别为１２个和３个，ＰＣＲ法的灵敏度为７０．６％，而涂片法仅为１７．６％，经统计学处理差异有显著性意义（Ｐ＜０．０１）。３８个关节中，ＰＣＲ法与涂片法检出的阳性数分别为１４个和５个，ＰＣＲ法和涂片法的阳性检出率分别为３４．２％和１３．２％，经统计学处理两者差异亦有显著性意义（Ｐ＜０．０５）。结论：应用ＰＣＲ技术检测关节液中的结核杆菌可以早期诊断关节结核并具快速、特异、灵敏等优点。
Objective:To diagnose joint tuberculosis early Methods:PCR was used to detect Mycobacterium tuberculosis in joint fluid from 33 patients(38 joints) suspected to have joint tuberculosis.The obtained joint fluid was detected by acid fast staining in the meantime The final diagnosis was established on pathological study which is the 'gold standard'.Results:PCR positive and smear positive numbers were 12 and 3 respectively in 17 joint tuberculosis of all 38 joints diagnosed by pathological study.The sensitivity of PCR and acid fast staining were 70.6%(12/17) and 17.6%(3/17)respectively( P <0.01) The positive detection numbers were 13 and 5 respectively for PCR and smear in all 38 joints.The positive detection rates of two assays were 34 2%(13/38) and 13 2%(5/38) respectively( P <0 05).Conclusion:Sensitivity and positive detection rate of PCR were higher than those of acid fast staining.PCR is a reliable sensitive and specific procedure for early diagnosis of joint tuberculosis.
出处
《滨州医学院学报》
1999年第2期117-119,共3页
Journal of Binzhou Medical University
关键词
结核
关节
聚合酶链反应
诊断
tuberculosis
joint
polymerase chain reaction(PCR)
diagnosis
