摘要
目的探讨碱性成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ）对重组人骨形成蛋白２（ｒｅｃｏｍｂｉｎａｎｔｈｕｍａｎｂｏｎｅｍｏｒｐｈｏｇｅｎｅｔｉｃｐｒｏｔｅｉｎ２，ｒｈＢＭＰ２）诱导骨形成的影响，评价多孔聚乳酸（ｐｏｌｙｌａｃｔｉｃａｃｉｄ，ＰＬＡ）复合人工骨修复骨缺损的作用。方法在５６例下颌骨缺损的兔模型中分别植入ＰＬＡ／ｒｈＢＭＰ２／ｂＦＧＦ、ＰＬＡ／ｒｈＢＭＰ２、ＰＬＡ／ｂＦＧＦ、ＰＬＡ，并设空白对照。植入后２、４、８周行Ｘ线摄片和组织学观察骨缺损区骨形成的情况。结果３种材料复合物较两种材料复合物成骨更好，而后者又较单一的ＰＬＡ成骨好，所有含骨生长因子的植入组成骨量与对照组比较差异均有显著性（Ｐ＜０．０５）。结论ＰＬＡ具有良好的生物相容性和可吸收性，有骨传导性能；ｂＦＧＦ可促进ｒｈＢＭＰ２在骨缺损愈合过程中的骨诱导作用；ＰＬＡ／ｒｈＢＭＰ２／ｂＦＧＦ复合材料是一种有临床应用前景的骨移植材料。
Objective To study the effect of basic fibroblast growth factor(bFGF)
on bone formation induced by recombinant human bone morphogenetic protein2(rhBMP2) in
repair of mandibular defects in rabbits. Methods 56 adult domestic rabbits were classified into
five groups. The mandiblar threewall defects(1.5 cm0.5 cm ,full thick) were created in right
sides. The defects were implanted with various grafts: PLA/rhBMP2/bFGF, PLA/rhBMP2,
PLA/bFGF, PLA and no implant as control group. At 2, 4 and 8 weeks after operation, the
effectivenesses of defect repaire were observed by histological and roentgenographic analysis.
Results The composite consisting of three materials stimulated more bone formation than of
two materials, and the latter was more than single PLA(at 4 weeks postoperation, P<0.05). All
experimental groups,which had one or two growth factors, were significantly higher compared
with control group in every stage of postimplantation. Conclusion PLA has good
biocompatibility, absorbability and osteoconductibility, rhBMP2 and bFGF are cooperated in
bone healing process, and PLA/rhBMP2/bFGF is a promising bone substitute in clinical uses.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
1999年第3期168-171,共4页
Chinese Journal of Stomatology
