摘要
设计不同的小豆根瘤菌及菌液浓度、侵染时间和接种方式接种小豆推广品种京农5号和京农6号,研究其对小豆结瘤数的影响。根瘤菌BAU73042菌液浓度0.6 OD接种,单株平均结瘤数最多;BAU11017菌株菌液浓度0.2 OD接种,单株平均结瘤数最多。BAU73042侵染京农6号15 min单株结瘤数最多,BAU11017侵染京农5号45 min单株结瘤数最多。菌液浇灌接种方式优于菌液侵染接种;不同品种接种不同根瘤菌的结瘤反应不同。该研究为小豆固氮研究及利用和根瘤突变体筛选鉴定奠定了基础。
Different concentrations of Rhizobia from azuki bean, infection time and inoculation methods were designed to study their effects in the number of nodules in azuki bean. The results showed that the maximum nodulations were generated with inoculating BAU73042 strain of 0.60D and BAU11017 of 0.2 OD. The most of nodules inoculated by BAU73042 and BAU11017 were produced at 15 min and 45 min in Jinnong 6 and Jinnong 5 respectively. More nodules were obstained in pouring Rhizobia than infec- ting. Different strains resulted in nodulating differences in different azuki bean varieties.
出处
《北京农学院学报》
2010年第3期1-4,共4页
Journal of Beijing University of Agriculture
基金
北京市教委人才强教引进人才项目(PXM2007-014207-04453)
北京市教委科研计划面上资助项目(KM201010020004)
关键词
小豆
根瘤菌
接种
结瘤
azuki bean（Vigna angularisi）
Bacterium Rhizobium
inoculation
nodules
