摘要
目的观察脐血间充质干细胞移植治疗糖尿病足的疗效和安全性。方法将脐血间充质千细胞多点注射到自愿接受干细胞移植的2例患者病变下肢，细胞数（3～7）×10^7／L，每点0．3～0．5ml，每点间距3cm×3cm。肌肉组织丰富的部位可分层注射。术后第1天至3个月定期观察患者临床症状及各项指标的变化并进行综合分析。结果脐血间充质干细胞移植后3个月进行评价，2例患者下肢疼痛均明显缓解、皮温升高、皮肤凉感消失、间歇性跛行明显改善和足部溃疡愈合。踝肱指数、经皮氧分压较前有明显升高，血管造影显示治疗后病变下肢均有丰富的侧支血管生成。移植后2例患者均未出现严重并发症和明显不良反应，均未行截肢术。结论脐血间充质干细胞移植治疗糖尿病下肢缺血性血管病是一种安全、有效的手段．可使一部分患者免除截肢，改善其生活质量。
Objective To observe the clinical effect and safety of umbilical cord blood mesenchymal stem cell （UCB-MSC） transplantation in the treatment of diabetic foot. Methods UCB-MSC suspension （cell concentration （3 -7） × 10^7/L,0. 3 -0. 5 ml per point） was injected into multiple spots on affected lower limb with a 3 cm × 3 cm istance among each point. Demixing injection could be performed in regions with muhilayer muscles. Clinical symptoms and related index were routinely observed from the first day to three months after operation. Results After three months of UCB-MSC transplantation, pain of patients was relieved, skin temperature increased, intermittent claudication ameliorated, ulcer healed, ankle-braehial index and transeutaneous partial pressure of oxygen increased. The lower extremity lesions showed an abundant collateral vessel formation after the treatment in 2 patients by angiography. Both patients had no severe complications and adverse reactions, none underwent amputation. Conclusions Umbilical cord blood mesenchymal stem cell transplantation is a safe and effective treatment of diabetic lower limb ischemic disease, which can exempt the patient from amputation and improve their quality of life.
出处
《中国综合临床》
2010年第9期918-920,共3页
Clinical Medicine of China
关键词
糖尿病足
移植
脐血间充质干细胞
Diabetic foot
Transplantation
Umbilical cord blood mesenchymal stem cell
