摘要
随机选取6只SD大鼠(Rattus norvegicus)和7只昆明小鼠(Mus musculus),用免疫组织化学单标和双标法检测其空肠及肾ATP7B与PCNA的表达,并分析表达的相关性。结果发现,对于大鼠及小鼠,ATP7B主要表达于小肠腺与空肠上皮的纹状缘、近腔面和近基底部,肾小管与集合管;PCNA在空肠腺及小肠绒毛中轴的结缔组织中表达,在肾小管、集合管及肾小球的少数细胞表达;ATP7B与PCNA虽在空肠上皮、肠腺、肾小管和集合管有共表达现象,但二者在大鼠与小鼠空肠及肾的免疫反应阳性物积分光密度间均无显著相关性(P>0.05)。提示ATP7B与PCNA在正常大鼠与小鼠空肠及肾的表达相似,ATP7B的表达与组织增殖活跃程度间的相关性不明显。
The jejuna and kidneys were randomly taken from 6 SD rats（Rattus norvegicus）and 7 Kunming mice （Mus musculus）. The expression of ATP7B and PCNA was observed by immunohistochemistry technique,and the relevance of ATP7B and PCNA expression was analyzed. The results showed that,in both rat and mouse, ATP7B was mainly expressed in small intestinal glands,striated border,near-cavity surface and near-basement surface of intestinal epithelial cells,as well as renal tubule and collecting duct. PCNA was strongly expressed in small intestine glands and the connective tissues. In kidney,PCNA was expressed in a few cells of renal tubule, collecting duct and glomerulus. Although both ATP7B and PCNA were expressed in the jejunum and kidney in both species,there was no significant correlation between integral optical density of ATP7B and PCNA immunoreactive positive substances （ P〉0. 05 ）. In jejunum and kidney,the expression of ATP7B had no relationship with the level of cell proliferation.
出处
《动物学杂志》
CAS
CSCD
北大核心
2010年第4期156-163,共8页
Chinese Journal of Zoology
基金
2007年新乡医学院大学生科研资助课题
