摘要
为了探讨肾脏细胞凋亡在糖皮质激素治疗狼疮性肾炎机制中的作用，采用甲基泼尼松龙（ＭＰＳ）和近交系纯合子ＭＲＬ－ｌｐｒ／ｌｐｒ自发狼疮小鼠进行体内实验，利用计算机图像分析系统观察了ＭＰＳ对狼疮小鼠肾脏细胞凋亡的诱导，并应用逆转录－巢式聚合酶链反应和Ｎｏｒｔｈｅｒｎ杂交技术观察了ＭＰＳ对哺乳动物凋亡相关基因白细胞介素－１β转化酶（ＩＣＥ）表达的影响。结果表明，ＭＰＳ冲击治疗组小鼠肾小球系膜区细胞的凋亡数目显著高于对照组（Ｐ＜０．０５），肾小管间质细胞和肾血管周浸润细胞的凋亡数目也明显高于对照组（Ｐ均＜０．０１），治疗组肾脏细胞ＩＣＥ基因的表达明显增强。研究提示，ＭＰＳ能够诱导狼疮小鼠的肾脏细胞发生凋亡，ＩＣＥ基因可能参与了狼疮小鼠肾脏细胞的凋亡机制。
MPS pulse therapy is quite effective for the treatment of lupus nephritis. At present, no research work has been reported on the relationship between MPS therapy and apoptosis and ICE expression in kidneys with lupus nephritis. We investigated the effects of MPS on the number of apoptotic cells and expression of ICE mRNA in the kidneys of fourmonth old MRL lpr/lpr mice, by means of image analysis system, RT PCR and Northern blot. The results showed that in MPS group, the number of apoptotic cells in glomerular mesangium increased significantly, as compared with that of a control group ( P < 0.05 ). Besides, the number of apoptotic cells in both the peri tubule cells and peri vascular infiltrating cells was also higher in MPS group than in the control group (both P <0.01). Furthermore, higher expression of ICE mRNA was found in the treated group than in the control group. Our results suggest that MPS may induce both apoptosis and ICE mRNA expression in kidneys with nephritis, implying that apoptosis may be involved in the outcome of lupus nephritis treated with MPS.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1997年第2期79-82,共4页
Chinese Journal of Internal Medicine
基金
国家自然科学基金青年基金
优秀中青年人才专项基金资助
