摘要
目的探讨脱细胞软骨基质对软骨细胞体外生长的影响。方法以脱细胞异体兔耳软骨基质为支架材料,以脱细胞异体兔耳软骨膜为对照,体外种植兔耳软骨细胞进行常规培养,观察软骨细胞的生长状况和增殖情况。结果软骨细胞进入脱细胞异体软骨基质构架裸露的空穴,生长旺盛。而在脱细胞异体软骨膜上,软骨细胞不易生长。结论支架材料的表面性状对细胞生长有显著影响,异体脱细胞软骨基质可提供适宜于软骨细胞生长的良好环境,有可能发展成为软骨组织工程的一种天然支架材料。
Objective To observe the effect of chondrocyte culture with natural ECM made from allogenous acellular cartilage.Methods The allogenous acellular cartilaginous matrix was used as a scaffold in chondrocyte cultrue in vitro,comparing with the acellular perichondrium.The growth and regeneration of the chondrocytes in vitro were observed.Results The chondrocytes seeded on the acellular cartilagi- nous matrix grew well while the chondrocytes on the acellular perichondrium matrix grew poorly.Conclu- sion The surface condition of the scaffold has a notable influence on the growth of the cells.The alloge- nous acellular cartilaginous matrix may provide a suitable condition for chondrocytes,and it has the possi- bility to be developed as a natural scaffold for tissue engineering.
关键词
组织工程学
软骨细胞
脱细胞软骨基质
细胞培养
Tissue engineering
Chondrocyte
Acellular cartilaginous matrix
Culture in vitro
