摘要
我们这项研究旨在寻求一种能够避免组织移植和人工材料的缺点，又能获得自体组织移植相同的效果的移植材料。选用１０只纯种新西兰大耳白兔，异体兔耳软骨细胞外基质（ＥＣＭ）。兔耳软骨缺损分别以下列四组方式修复：①兔耳软骨ＥＣＭ＋兔耳软骨骨膜，②兔耳软骨ＥＣＭ，③兔耳软骨膜，④空白。术后６、１２、２４、４８周分别切取修复区域标本，做ＨＥ、奥新蓝两种染色。结果显示，ＥＣＭ植入体内后６周有轻度的炎症反应，术后１２周炎症异物反应消失，ＥＣＭ周边无包裹现象，术后１２周骨膜细胞长入ＥＣＭ，但细胞排列不规则。术后２４周、４８周ＥＣＭ仍然存在，无吸收现象，软骨膜与ＥＣＭ覆着处细胞长入ＥＣＭ，排列较１２周时规则，呈奥新蓝反应强阳性。结论：①ＮＥＣＭ可以作为组织充填物而长期存在并有较好的组织亲和性和相容性，因此有希望成为新一代的组织替代物。②ＮＥＣＭ可以诱导软骨膜细胞向其中生长而重建软骨，因此软骨ＮＥＣＭ有可能是复合生长因子。③软骨膜可以作为软骨ＮＥＣＭ复制软骨组织的细胞来源。④根据本实验观察一年的结果证明应用兔耳软骨ＥＣＭ，可以复制出新的软骨。
In order to find out some new substitution for autotransplantation yet without the disadvantages of tissue transplantion and artificial material implantation, the Extracellular Matrix(J.Y Life Tissue Engineering Ltd.) was used for new tissue regeneratin. In the study 1cm×1cm defect was made in both ear of 10 New Zealand white rabbits. the defects were treated by one of the following 4 models: ①ECM+perichondium, ②ECM, ③perichondium, ④blank control. The specimens were obtained at sixth、twelfth、twenty-fourth, and fourty-eighth week postoperatively and were stained with hematoxy lin-eoscn and aldehyde fusion-alcian blue, respectively. The result revealed that there was a slight inflammatory reaction surround the ECM 6 weeks after they were implanted and this phenomenon disappeared at the twelfth week postoperatively. No capsule was found around the implanted ECM. In Group Ⅰ, the perichondial cells were found to immigrate into ECM from the twelfth week on postoperatively. The new cells found in the ECM presented postive reaction to aldehyde fusion-alcian blue stain suggesting that there were living chondial cells in the implanted ECM. From the experiment, it could be concluded that (1) Nature ECM can be implanted in the body for a long time with good histo-compatibility, it may be a new tissue substitute in the futrue. (2)natrue ECM can induce the perichondial cell to immigrate into it, this phenomenon suggest that the N-ECM might be a complex growth factor. (3)Perichoudiam can serve as the resource of chondial seed cells. (4)The rabbit ear cartilage can be regenerated with allogenic chondial ECM.
出处
《实用美容整形外科杂志》
1997年第5期228-231,共4页
Journal of Practical Aesthetic and Plastic Surgery
关键词
组织工程
软骨
再生
天然细胞外基质
Tissue Engineering
Cartilage
regeneration
Natrue ECM
