摘要
土壤盐碱化现已成为危害农业发展和生态环境的全球性问题,培育和鉴选棉花耐盐品种是合理开发利用盐碱地的有效途径。本研究选用25份耐盐(包括耐和抗)和23份盐敏感棉花种质为实验材料,采用SSR技术,展开了棉花耐盐性鉴定技术的有关研究。从DNA快速提取到PCR扩增和产物检测以及多标记组合鉴定等环节进行分析探讨,初步制定了一套适于棉花耐盐性分子鉴定的方法,即多标记组合鉴定法。并用11份材料对该方法进行了验证,结果表明和0.4%盐量胁迫法的鉴定结果的相符率达90.91%。初步研究结果表明多标记组合鉴定法可用于棉花耐盐分子标记辅助鉴定。
Soil salinization has become a serious global problem affecting the agricultural development and the ecological environment.It is an effective way to farm saline land and to enhance the sustainable agricultural development by developing,identifying and screening the salinity tolerant varieties of cotton.25 salinity tolerant and 23 salinity sensitive cotton accessions were used in this study to identify salinity tolerance of cotton by SSR markers.A new set of preliminary methods system,called SSR multi-markers salinity-identification method,was initially established to identify salinity tolerance of cotton by the standardization of the whole process of DNA extraction,PCR amplification,amplification products detecting,and marker-combination.Another 11 materials were used to testify this method,which showed the coincidence of 90.91%in consistence with the identification result of 0.4% NaCl identification method,which showed that the multi-markers identification method was proved to be used to identify the salinity tolerance of cotton germplasm.
出处
《分子植物育种》
CAS
CSCD
2010年第5期891-898,共8页
Molecular Plant Breeding
基金
转基因生物新品种培育科技重大专项(2008ZX08005-004)
“十一五”国家科技支撑计划(2006BAD13B04-1)共同资助
关键词
棉花
耐盐
SSR
标记组合
Cotton
Salinity tolerance
SSR
Multi-markers
