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重组人乳铁蛋白对不同卵巢癌细胞株增殖影响的实验研究 被引量：2

Study on inhibition effect of recombinant human lactoferrin in vitro on human ovary carcinoma cell lines proliferation
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摘要 目的:探讨重组人乳铁蛋白(rhLF)对体外培养的3株人卵巢癌细胞的影响。方法:体外培养SKOV3、HO-8910、HO-8910PM,用MTT法评价rhLF对三者的生长抑制作用。结果:重组人乳铁蛋白对3株细胞均有抑制作用并呈剂量依赖关系,对SKOV3的抑制较另两株显著,不同浓度的rhLF对SKOV3增殖的抑制率达40.95%、62.03%、72.63%,实验对照组细胞无明显的抑制效果。结论:重组人乳铁蛋白可以有效抑制卵巢癌细胞的体外增殖,为其作为新的肿瘤生物治疗剂奠定了理论基础。 Objective：To explore the effect of lactoferrin on proliferation of the cultured SKOV3 cells in vitro.Methods：To cultivate SKOV3,HO-8910,HO-8910PM cells in vitro.. MTT assay were used to evaluate the growth inhibition effect of lactoferrin on SKOV3.Results：Lactoferrin significantly inhibited the proliferation of three cells in a dose-dependent manner,the inhibitor rate of SKOV3 was 40.95%,62.03%,72.63% in different concentration,respectively,but had no obvious inhibition on the control cells.Conclusion：Lactoferrin can effectively inhibit the proliferation of Ovary cancer cells,and lay a foundation for exploitation of a new chemistry agent.
作者 仇志琴 虞丰 顾志群 何琳 周晔 蔡蓉
机构地区 南京医科大学附属无锡第二人民医院妇科
出处 《中国妇幼保健》 CAS 北大核心 2010年第26期3787-3789,共3页 Maternal and Child Health Care of China
基金 江苏省卫生厅重点开放项目〔WK0511〕
关键词 乳铁蛋白 卵巢癌 细胞增殖 抑制率 Lactoferrin Ovary cancer Cell proliferation Inhibitor rate
分类号 R737.31 [医药卫生—肿瘤]
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参考文献8

  • 1Ward PP, Uribe - Luna s, Conneely OM. Lactoferrin and host defense [J] . Biochem Cell Biol, 2002, 80:95.
  • 2Cumberbatch M, Dearman R J, Uribe - Luna S et al. Regulation of epidermal Langerhans cell migration by lactoferrin[J] . Immunology, 2000, 100 (1): 21.
  • 3Bezauh J, Bhimani R, Wipmvnick J et al. Human lactoferrin inhibits growth of solid tumors and development of experimental metastases in mice[J] . Cancer Res, 1994, 54 (9): 2310.
  • 4Varadhaehary A, Wolf JS, Petrak K et al. Oral lactoferrin inhibits growth of established tumors and potentiates conventional chemotherapy [J] . IntJCancer, 2004, 111 (3): 398.
  • 5XiaoY, Monitto CL, Minhas KM et al. Lactoferrin down regulates GI cyclin- dependent kinases during growth arrest of head and neck cancer cells [J] . Clin Cancer Res, 2004, 10 (23) : 8683.
  • 6Damiens E, E1Yazidi I, Mazurier Jet al. Lactoferrin inhibits GI cyciin -dependent kinases during growth arrest of human breast carcinoma [J] . J Cell Biochem, 1999, 74 (3) : 486.
  • 7Hayes TG, Falehook GF, Varadhaehary GR et al. Phase I trial of oral talactoferrin alfa in refractory solid tumors[J] . invest New Drugs, 2006, 24 (3): 233.
  • 8Jonasch E, Stadler WM, Bukowski RM et al. Phase 2 trial of talactoferrin in previously treated patients with metastatic renal cell carcinoma [J] .Cancer, 2008, 113 (1): 72.

同被引文献18

  • 1黄玉政,成勇,柏亚军,冯亚杰.人乳铁蛋白转基因小鼠杂交选育的研究[J].动物医学进展,2007,28(6):39-43. 被引量：6
  • 2Vanderstate BWA, Beljaars L, Molema G, et al. Antiviral activities of lactoferrin [ J ]. Antiviral Res,2001,52 (3) :225.
  • 3Baker EN, Baker HM. Molecular structure. Binding properties and dy- namics of laetoferrin[ J]. Cell Mol Life Sci,2005,62 (22) :2531.
  • 4Mckeown S, Lundy FT, Nelson J, et al. The cytotoxic effects of human neutrophil peptide-1 (HNP1) and lactoferrin on oral squamous cell carcinoma( OSCC ) invitro[ J] Oral Oncology,2006,42(7) :685.
  • 5Sakai T, Banno Y, Kato Y, et al. Pepsin - Digested Bovine Lactoferrin Induces Apoptotic Cell Death With JNK /SAPK Activation in Oral Cancer Cells[ J]. J Pharm acol Sci,2005,98( 1 ) :41.
  • 6Wolf JS, Li GY, Varadhachary A, et al. Oral laetoferrin results in T cell-dependent tumor inhibition of head and neck squamous cell car- cinoma in vivo [J]. Clin Cancer Res ,2007,13 (5) :1601.
  • 7Norrby K, Mattsby-Bahzer I, Innocenti M, et al. Orally administered bovine lactoferrin systemically inhibits VEGF (165)-mediated angio- genesis in the rat[ J]. Int J Cancer,2001,91 (2) :236.
  • 8Bezauh J, Bhimani R, Wipmvnick J, et al. Human lactoferrin inhibits growth of solid tumors and development of experimental metastases in miee[J]. Cancer Res,1994,54(9) :2310.
  • 9Varadhaehary A, Wolf JS, Petrak K, et al. Oral lactoferrin inhibits growth of established tumors and potentiates conventional ehemothera- py[J]. Int J Cancer,2004,111 (3) :398.
  • 10Hayes TG, Falchook GF, Varadhaehary GR, et al. Phase I trial of oral talaetoferrin alfa in refractory solid tumors [J] Invest New Drugs, 2006,24 (3) :233.

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中国妇幼保健
2010年 第26期

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