摘要
目的观察妊娠高血压综合征（pregnancy induced hypertension syndrome，PIH）患者胎盘细胞凋亡的情况。方法采用DNA缺口原位标记Tunel’s技术，测定50例PIH患者和30例正常妊娠妇女胎盘细胞凋亡指数，并用电镜观察细胞的超微结构改变。结果PIH胎盘绒毛滋养细胞、合体滋养细胞、蜕膜细胞凋亡指数分别为（5．46±2．60）％、（7．48±3．90）％、（5．73±2．89）％，对照组分别为（3．95±1．75）％、（5．42±1．96）％、（4．08±1．99）％，二者差异均有统计学意义（P〈0．05）；PIH并发症患者电镜显示PIH并发症患者胎盘滋养细胞与合体滋养细胞均有不同程度的异常形态变化。结论细胞凋亡是PIH并发症不良妊娠结局的重要因素之一，是影响胎盘功能的直接原因。
Objective To Observe the placental apoptosis in patients with pregnancy induced hypertension （PIH） with complications. Methods TDT - mediated DUTP nick and labeling（ Tunel） technique was used for 50 human placental samples with PIH with complications and 30 normal placental samples to exams apoptosis index in cytotropho - blast syncytiotrophoblast and decidual cells to observe these samples under the electronic microscope. Resuits The cytotrophoblast, syncytophoblast, decidual cell in PIH with complications group were （ 5.46±2.60 ） %, （ 7.48±3.90 ） %, （ 5.73±2.89 ） %, respectively. The cytotrophoblast, syncytophoblast, decidual cell in control group were（3.95±1.75 ） %, （ 5.42±1.96 ） %, （ 4.08±1.99 ） %, respectively. The apoptosis indices in placental cytotrophoblast, syncytophoblast, decidual cell were higher in PIH group than in control group. The significant morphologies of apoptosis were found under the electroni cmicroscope. Conclusion Apoptosis is a direct reason on effect of polacental functions and also an important factor on critical pregnanc result in PIH with complications.
出处
《医学新知》
CAS
2010年第4期269-270,272,共3页
New Medicine
关键词
妊娠高血压综合征
细胞凋亡
胎盘
pregnancy induced hypertension
apoptosis
placenta
