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SARS S1基因对番茄的遗传转化与转基因番茄植株的获得 被引量：1

Genetic Transformation of Tomato with SARS S1 Gene and Production of Transgenic Tomato Plants
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摘要 利用DNA直接导入法将含有SARS S1基因的植物表达载体pCS1导入农杆菌LBA4404中,并通过农杆菌介导的叶盘转化法得到12株再生小苗。PCR、Southern杂交检测结果证实,有6株为真正的转基因植株。 The plant expression vector pCS1 was introduced into Agrobacterium tumefaciens LBA4404 by direction introduction of alien DNA,and 12 regenerated plantlets were obtained by Agrobacterium-mediated leaf disk transformation method. PCR and Southern blot analysis suggest that 6 regenerated plantlets are transgenic plants.
作者 谭琳 康由发
机构地区 海南医学院生化教研室 中山大学中山医学院 海南大学应用科技学院
出处 《生物技术通报》 CAS CSCD 北大核心 2010年第10期131-134,共4页 Biotechnology Bulletin
关键词 SARS S1基因 转化转基因番茄 SARS S1 gene Transformation Transgenic tomato
分类号 Q943.2 [生物学—植物学]
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参考文献14

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生物技术通报
2010年 第10期

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