摘要
目的:研究耐力训练对大鼠血清护骨素(OPG)、破骨细胞分化因子(可溶性)sRANKL及骨代谢生化标记物、骨密度(BMD)和骨量(BMC)的影响。方法:20只6周龄大鼠随机分成2组:①对照组10只,不运动,正常饮食及生活;②训练组10只,进行12周跑台运动训练,每天训练1次,坡度为0,第1周8m/min×10min,第2周15m/min×45min,第3周及以后25m/min×60min。实验结束后,所有大鼠安静处死并测定血清维生素D(3VD3)、骨钙素(OC)、骨碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)及OPG、sRANKL。同时测定股骨长度和胫骨、腰椎BMD、BMC。结果:经过12周运动训练,运动组大鼠股骨显著长于对照组(P<0.05),胫骨、腰椎BMC明显高于对照组(P<0.05,P<0.01),而BMD无显著性差异。运动组大鼠血清OPG水平明显高于对照组(P<0.05),而sRANKL明显低于对照组(P<0.01)。与此相对应的是,运动组OC和ALP明显高于对照组(P<0.05),而TRAP显著低于对照组(P<0.05)。运动组血清维生素D也显著高于对照组(P<0.05)。结论:运动导致OPG/sRANKL比例升高可能是耐力训练促进生长期大鼠骨量增加的重要因素。
Objective To investigate effects of endurance exercise on the changes in osteoprotegerin（OPG）,the receptor activator of nuclear factor κB ligand （RANKL）,and bone biomarker,bone mass density （BMD）,and bone mass content （BMC）. Methods Twenty 6-week old rats were equally randomized into two groups：①control group （Sed group）,fed with normal forage and underwent regular physical activity. ②12-week training group（Ex group）, underwent treadmill running once a day （8m/min×10min for the first day,15m/min×45min for the second day,and 25m/min×60min until the end of experiment）for 12 weeks. After completion of experiment,all rats were killed and blood bone samples were collected to determine serum vitamin D（3VD3）,OPG,sRANKL,OC,ALP,and TRAP,and BMD and BMC. Results It was shown that longer femur length（P 0.05）,and tibial length（P 0.05）,and thicker lumbar spine（P 0.01）and higher BMC revealed in Ex group as compared with that in Sed group. Comparing to the Sed group,serum OPG was higher（P 0.05）and sRANKL was lower（P 0.01）in Ex group. Rats in Ex group had higher serum OC（P 0.05）,ALP（P 0.05）,and VD（P 0.05）,but lower TRAP（P 0.05）as compared with that in Sed group. Conclusions Exercise-induced increased ratio of OPG/sRANKL could be an important factor in the promoting BMC in rats during growing period.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2010年第5期560-562,共3页
Chinese Journal of Sports Medicine
