摘要
目的:观察黄连对小鼠红细胞氧化性溶血的影响并探讨相关机制。方法:采用乙酰苯肼(APH)诱导小鼠红细胞氧化性溶血模型,以伯氨喹啉为对照药,观察不同浓度的黄连对血浆游离血红蛋白、血清间接胆红素、全血网织红细胞、红细胞内脂质过氧化产物和抗氧化系统相关酶和蛋白以及全血总抗氧化能力的影响。结果:0.03 g.kg-1APH能引起正常小鼠血浆游离血红蛋白和血清间接胆红素含量及外周血网织红细胞数量显著升高,也能明显提高红细胞丙二醛(MDA)含量和降低红细胞超氧化物歧化酶(SOD)、6-磷酸葡萄糖脱氢酶(G6PD)活力、还原型谷胱甘肽(GSH)含量以及全血总抗氧化能力。0.058 g.kg-1伯氨喹啉能明显加重APH所引起的红细胞氧化性溶血和氧化系统破坏;0.6 g.kg-1黄连能明显降低血清间接胆红素含量,1.2 g.kg-1黄连尚能显著减少血浆游离血红蛋白量和全血网织红细胞数,同时明显降低红细胞MDA含量、增加SOD活力和GSH含量、提高全血总抗氧化能力。结论:黄连能一定程度减轻APH引起的小鼠红细胞氧化性溶血,其机制可能与降低脂质过氧化产物和提高红细胞抗氧化酶的量和能力有关。
Objective： To investigate the effect of Coptis chinensis on oxidative hemolysis of erythrocytes in mice.Method： Acetylphenyhydrazine（APH）-induced oxidative hemolysis of erythrocytes in mice were used.The contents of free hemoglobin of blood plasma,indirect bilirubin of serum,reticulocytes of blood,and malondialdehyde（MDA） of erythrocytes were measured.The activity of superoxide dismutase（SOD）,reduced glutathione hormone（GSH）,and glucose-6-phosp hate dehydrogenase（G-6-PD） of erythrocytes were also determined and the total-antioxygen capability（T-AOC） of blood was analyzed.Result： The levels or amount of free hemoglobin of blood plasma,indirect bilirubin of serum,reticulocytes of blood and MDA of erythrocytes were higher in APH（0.03 g·kg^-1-induced mice than normal mice.The activity or content of SOD,GSH and G-6-PD was lower in APH-induced mice than in normal mice.Primaquine（0.058 g·kg^-1 could aggravated the degree of elevated hemolysis of erythrocytes in APH-induced mice.C.chinensis（0.6 g·kg^-1 could deprssed significantly the elevated levels of indirect bilirubin in serum.The levels of free hemoglobin of blood plasma,indirect bilirubin of serum,reticulocytes of blood,the production of SOD and GSH and T-AOC were also decressed by C.chinensis（0.6 g·kg^-1.Conclusion： C.chinensis suppressed the degree of hemolysis of erythrocytes in APH-induced mice due to the suppression of the production of lipid peroxidation and increasing of the activity of antioxidase of erythrocytes.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第18期2449-2452,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30371785)
关键词
黄连
小鼠
红细胞
氧化性溶血
Coptis chinensis
mouse
erythrocytes
oxidative hemolysis
