摘要
比较了4类(5种)不同大孔树脂对油菜籽原花青素的吸附与解吸性能,从中筛选出适用于油菜籽原花青素纯化的两种树脂,它们均属弱极性树脂。对其吸附状态及适用性进行探讨后,给出了初步的纯化工艺。结果表明,弱极性的AB-8和HPD450大孔吸附树脂对油菜籽原花青素的吸附量、吸附速率及解吸性能等均优于其它3种大孔树脂。在本实验条件下单独使用AB-8大孔树脂柱制备的油菜籽原花青素的纯度略高于HPD450大孔树脂制备的原花青素,但收率略低。经工艺优化,先采用HPD450柱,后采用AB-8柱进行分步串联纯化,则获得了更好的效果:获得原花青素干粉纯度达84%,收率为62.5%。采用50%HPD450与50%AB-8(v/v)混合树脂柱,则经一次纯化,纯度达80.3%,收率为75.2%,有一定工业价值。
The characteristics of absoption and desorption of Four classes（five kinds） of macroporous resins in the solution of rapeseed proanthrocyanidin were compared.The results indicated that the weak polar macroporous resins AB-8 and HPD450 were better than the other three macroporous resins in some characteristics of absorption and desorption.In this experiment,the purity of rapeseed proanthrocyanidin prepared only by AB-8 macroporous resin column was higher than only by HPD450 column.However,the yield was lower.Using two steps of purification（first HPD450 column and second AB-8 column） obtained better results.The yield and purity were 62.5%and 84%,respectively.After using mixed resin column（50%HPD450 and 50%AB-8）,the yield and purity were 75.2% and 80.3%,respectively.The last results might be used in industry.
出处
《中药材》
CAS
CSCD
北大核心
2010年第8期1324-1329,共6页
Journal of Chinese Medicinal Materials
基金
江苏省科技厅农业重点攻关项目(BE2006310)
关键词
油菜籽
原花青素
大孔树脂
吸附
解吸
纯化
Rapeseed coat
Proanthrocyanidin
Macroprous resin
Adsorption
Desorption
Purification
