摘要
作者采用α-^(32)P-dCTP标记的v-abl癌基因探针与7例慢粒白血病细胞DNA的PvuⅡ、Hind Ⅲ和Bg 1 Ⅱ酶切片段杂交。结果发现其中3例慢粒病例的Pvu Ⅱ和Hind Ⅲ酶切杂交图谱与正常对照相比有明显变化,且Pvu Ⅱ酶切位点有多处改变。表明在慢粒白血病中,Ph染色体重排亦可累及 c-abl癌基因内部,并可能有多次多点重排发生。
The authors analyzed the PvuⅡ, HindⅢ and BgⅢ restriction enzyme mappings of seven cases of chronic myeloid leukemia, using a v-abl probe labeled by α-^(32)P-dCTP. The results showed that the PvuⅡ and HindⅢ mappings of three cases among the seven had been remarkably altered and the multiple changes of PvuⅡ sites had been observed, as compared with the normal subjuct. Therefore, the authors predicted that Ph chromasomal rearrangement in this kind of chronic myeloid leukemia could involve the inside of c-abl oncogene and might be a multiple rearrangement as well.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1990年第1期42-44,共3页
Progress In Biochemistry and Biophysics
基金
国家科技攻关项目基金
关键词
白血病
c-abI癌基因
基因重排
chronic myeloid leukemia, c-abl oncogene, gene rearrangement, v-abl probe, Southern blot hybridization
