摘要
采用半连续培养技术研究了假单胞杆菌(Pseudomonas)DM1在二氯甲烷降解过程中的微生物动力学。高浓度底物二氯甲烷和高浓度产物氯化物对DM1的生长和底物的消耗具有严重的抑制效应。反应的最佳浓度条件是:CH_2Cl_2 1.2mmol/L,氯化物<100mmol/L。当二氯甲烷或氯化物浓度高于其极限浓度时,细菌失活并自溶,μ≤0。对于具有严重底物抑制的发酵过程,特别是以有毒化合物为唯一碳源的生物降解过程,本文提出一个新的动力学模型。由两项组成,第一项反映了传统的Monod类型的可逆络合机理,第二项表示有毒物质对生物体代谢作用的不可逆抑制效应。该模型比其他传统模型能更好地描述二氯甲烷的生物降解过程。
The microbial kinetics of Pseudomonas sp. strain DM1 during dichloromethane biodegradation was studied by means of fed-batch culture technique. The growth of the bacteria and the consumption of the substrate were inhibited severely by the substrate CH_2Cl_2 and the product chloride at high concentrations. The optimal concentrations for reaction were CH_2Cl_2 1.2mmol/L and chloride<100 mmol/L. When the concentrations of CH_2Cl_2 and/or chloride were highet than their restricted values, the bacteria would die and autolyse, μ≤0. A modified kinetic model was proposed and can fit the experimental data, and is better than other traditional models. It consists of two terms, the first is due to the reversible Monod-type complexion mechanism and the second is regard as an irreversible inhibition effect of toxic compounds on the microbial metabolism.
出处
《生物工程学报》
CAS
CSCD
北大核心
1990年第1期56-62,共7页
Chinese Journal of Biotechnology
关键词
假单胞菌
二氯甲烷降解
动力学
Pseudomonas
CH_2Cl_2 degradation
substrate inhibition
product inhibition
inrreversible inhibitory effect
