摘要
以17β雌二醇(E2)诱导西伯利亚鲟幼鱼,取其血清样品后利用凝胶过滤层析进行分析,诱导组鱼血清中较对照组的多出一蛋白峰;收集浓缩该峰后经离子交换两步层析法进行分离,成功分离出西伯利亚鲟卵黄蛋白原(Vtg)。分离出的Vtg于7.5%的Native-PAGE凝胶电泳分析,得出其分子量为390 KDa;对Vtg氨基酸组成检测结果显示,谷氨酸、丙氨酸、赖氨酸、天冬氨酸和亮氨酸含量较高,分别为12.25%、10.15%、8.43%、8.37%和8.11%,但甲硫氨酸和组氨酸含量相比较低,分别为1.56%和2.42%。
Vitellogenin（Vtg） of Acipenser baerii serum was isolated,analysed and determined after being exposed to 17β-estradiol.Results showed that there was another protein peak comparing to the control group after being exposed to 17β-estradiol in Acipenser baerii serum by gel filtration chromatography.Vtg was effectively purified from the serum of Acipenser baerii by two steps of ion-exchange chromatography.The molecular weight of Vtg was 390 KDa through the Native-PAGE gel electrophoresis.Amino acid composition analysis showed that the Vtg was abundant in glutamic acid（12.25%）,alanine（10.15%）,lysine（8.43%）,aspartic acid（8.37%） and leucine（8.11%）,but the content of methionine and histamine was lower,1.56% and 2.42% respectively.
出处
《海洋渔业》
CSCD
北大核心
2010年第3期251-256,共6页
Marine Fisheries
基金
农业科技成果转化基金项目(2008GB23260408)
国家公益性行业(农业)科研专项(200903048-07)
国家高技术研究发展计划(863计划项目)(2008AA10Z227)
中央级公益性科研院所基本科研业务费专项资金资助项目(2007M01,2007M023)
关键词
西伯利亚鲟
卵黄蛋白原
凝胶过滤层析
离子交换层析
Acipenser baerii
vitellogenin
gel filtration chromatography
ion-exchange chromatography
