摘要
总结中药代谢组学研究中生物样品（尿液、血液）的前处理方法,主要包括：尿液中加入缓冲液提供相同的pH值和离子强度,保持代谢物化学位移的恒定。利用有机溶剂沉淀法除去血液中的大分子物质,增加色谱柱的性能和使用寿命。血液和尿液样品在进行GC-MS分析前要进行衍生化处理。同时,对尿液和血液的采集与储存方法进行了总结。
In order to reduce the chemical shift variation,the pH and the consistency of ionic strength were controlled by adding buffer to urine samples. In analysis of blood,efficient removal of macromolecules such as proteins by organic solvent precipitation before injection into an analytical LC column was important in enhancing the performance and extending column lifetime. Sample derivatization of blood sample and urine sample before GC-MS analysis was needed. And methods of collection and storage of urine and blood samples were also reviewed.
出处
《广东药学院学报》
CAS
2010年第4期434-437,共4页
Academic Journal of Guangdong College of Pharmacy
基金
广东省教育厅高水平科研项目(自然科学)(粤财教[2009]400号)
关键词
中药
代谢组学
样品前处理
traditional Chinese medicine
metabonomics
pretreatment
