摘要
目的研究胃癌组织表达胃泌素受体（ＧＲ）的特征，是临床应用ＧＲ拮抗剂调控胃癌细胞生长的基础。方法应用受体的放射配基结合分析法测定３４例胃癌及癌旁胃粘膜组织ＧＲ含量及亲和力（Ｋｄ）。结果３４例胃癌组织中，１６例胃癌组织ＧＲ阳性，其中２例属低亲和力ＧＲ；１４例属高亲和力ＧＲ，计高含量ＧＲ９例（３９．５４±１４．４３ｆｍｏｌ／ｍｇ蛋白）和低含量ＧＲ５例（６．０３±２．８３ｆｍｏｌ／ｍｇ蛋白）。胃癌组织表达高含量ＧＲ的平均含量较癌旁胃粘膜组织高。胃体癌表达高亲和力ＧＲ的阳性率为７７．８％，胃底贲门癌为５０．０％，而胃窦癌为２１．１％。晚期胃癌表达高亲和力ＧＲ的阳性率为５２．２％，早、中期胃癌为１０．０％。结论４７．１％胃癌组织能表达ＧＲ；高亲和力、高含量ＧＲ在胃体癌、胃底贲门癌中表达的阳性率高；晚期胃癌表达的ＧＲ多属高亲和力。
Objective To study the expression of gastrin receptor(GR) in gastric cancer as the theoretic basis for its regulation on the cancer growth. Methods Radioligand binding assay of receptor was used to determine the gastrin receptor (GR) and its affinity (Kd) in stomach cancer therapy in 34 patients with gastric cancer.Results 16 patients had positive GR, among which 2 were of low affinity GR, and 14 were of high affinity (Kd<1.0nM)GR, 9 cases had GR>10 fmol/mg protein(39.54+14.43 fmol/mg protein) 5 cases had GR<10 fmol/mg protein( 6.03+2.83 fmol/mg protein). High affinity GR expression was present in 77.8% and 50.0% in cancer of gastric body and fundus, respectively, but only 21.1% in cancer of gastric antrum. The positive rate of high affinity GR expression in Ⅲ、Ⅳ stages (52.2%) was higher than that in Ⅰ+Ⅱ stages (10.0%). Conclusion GR is expressed in 47.1% cases of gastric cancer. High affinity and high content GR were found in cancer of gastric body and fundus as well as in advanced gastric cancer.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1999年第2期104-106,共3页
Chinese Journal of Digestion
关键词
胃肿瘤
胃泌素类
受体
缩胆囊素
Stomach neoplasms Gastrin Receptors, cholecystokinin
