摘要
苯酚是进行高质量植物DNA提取常用的试剂,但苯酚对患病材料中病原检测及后续分子生物学试验的影响尚未见报道。本试验利用苯酚对健康和患枣疯病的枣树叶片中DNA进行提取,并进行了后续的病原检测和AFLP分析,结果表明(1)苯酚抽提获得的DNA质量及产率明显高于对照;(2)苯酚抽提处理对枣疯病病原的检测有直接影响,当病原含量较低时,加苯酚抽提后的DNA检测不到病原;(3)苯酚抽提处理与否获得的DNA对后续AFLP分析的扩增效果没有明显影响;但两者出现了有规律的差异条带,经分析此差异应该是由材料中有无病原引起的。本试验结果为寄主材料中病原检测及寄主与病原之间的互作等研究提供了有益参考。
Phenol is applied to plant DNA extraction commonly,but there was still no report about if phenol had influenced to pathogen detection and further molecular analysis.The healthy and diseased leaves infected by Jujube Witches′Broom（JWB） of Chinese jujube were used to extract total DNA and then to detect pathogen and carry out AFLP analysis.The results showed that（1）The quality and extraction rate of DNA obtained by using phenol was higher than CK;（2）Phenol had a directly influenced the detection of phytoplasma.The phytoplasma couldn＇t be detected in the leaves with lower amount of pathogen and treated by phenol;（3） Phenol had no influence to the amplified effects of AFLP.But there were obvious different bands between using phenol and without using phenol.It was considered that those different bands were caused by phytoplasma.Those results provided beneficial reference to the study of pathogen detection in host and the relation between pathogen and host.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2010年第5期640-644,共5页
Journal of Plant Genetic Resources
基金
河北省自然科学基金(C2009000534
08D020
C2005000267)
关键词
DNA提取
枣疯病
植原体
检测
苯酚
AFLP
DNA Extraction
Jujube Witches′ Broom
Phytoplasma
Detection
Phenol
AFLP
