摘要
目的:探讨丙泊酚对新生鼠大脑皮层神经元存活及凋亡的影响。方法:取新生1d内的SD(Sprague-dawley)大鼠的皮层神经元,培养6d,应用免疫细胞化学法及电子显微镜鉴定神经元,用不同浓度的丙泊酚作用神经元不同时间,四甲基偶氮唑蓝(MTT)法测细胞存活率,TUNEL染色法测细胞凋亡指数,Western-blot免疫印记检测Cleaved-caspase3蛋白的表达。结果:丙泊酚作用12h后,细胞存活率在6个组中无明显变化,作用24h及48h后,较高浓度组细胞存活率降低趋势明显。且较高浓度作用于神经元24h后凋亡指数及Cleaved-caspase3蛋白的表达均呈浓度依赖性增高(P<0.05)。结论:高浓度长时间丙泊酚作用于大脑皮层神经元可降低其存活力和促进细胞凋亡,这一结论对临床儿童麻醉用药有指导意义。
Objective：To investigate the effects of propofol on cell viability and apoptosis in primary cultured cortical neurons of newborn rats.Methods：Primary culture was prepared from newborn rat,and cultured for 6 days.Immunocytochemical and electronic Micoscopic methods were used to identify the cultured neurons.Cells were treated by propofol with different concentrations and at different times.The effects of propofol on neurons were evaluated by cell viability（MTT assay）and markers of apoptosis（TUNEL cell staining and Western-blot of Cleaved-caspase3）.Results：There were no significant differences in cell viability among the six groups after 12 h, whereas high concentration of propofol caused an increase in neuronal cell viability after 24 h and 48 h.Propofol incubation for 24 h triggered apoptosis on the neurons（P0.05）and up regulated Cleaved-caspase3 of neurons in a concentration dependent manner（P0.05）. Conclusion：Propofol treatment with long time and high concentration significantly decreased the cell viability and promoted the cell apoptosis.The present results offered guidance for children anesthesia in clinic.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2010年第8期1175-1178,共4页
Journal of Chongqing Medical University
