摘要
目的:探讨5-HT2A、5-HT7受体在雄性大鼠脊髓Onuf核不同运动神经元内分布及其与泌尿生殖功能中枢调控的关系。方法:成年SD大鼠10只(雄性8只、雌性2只),随机分为性别对照组(n=4,雌雄各2只)和逆行示踪组(雄性大鼠,n=6)。性别对照组取脊髓L5～6节段,切片后作5-HT2A或5-HT7受体免疫组化染色;逆行示踪组采用重组伪狂犬病毒株(PRV-152)分别注入右侧尿道外括约肌(EUS组;n=3)或右侧坐骨海绵体肌(IC组;n=3),4d后取L5～6节段,切片后作5-HT2A或5-HT7受体双标记荧光免疫组化染色。分别在光镜或激光共聚焦显微镜下观察5-HT2A、5-HT7受体在Onuf核的分布情况。结果:5-HT2A受体的免疫产物主要位于Onuf核背外侧核的内侧,5-HT7受体则主要位于背外侧核的外侧;雄性大鼠5-HT2A、5-HT7受体在Onuf核的表达明显强于雌性大鼠。免疫荧光双标记显示5-HT2A受体的免疫标记主要分布于支配IC的运动神经元周围,5-HT7受体主要分布于支配EUS的运动神经元。结论:5-HT2A、5-HT7受体不均匀分布在Onuf核支配不同的盆底横纹肌的运动神经元。5-HT2A受体可能主要参与性反射的调节,5-HT7受体则可能主要参与泌尿反射的调控。
Objective：To investigate the distribution of serotonin 5-HT2A and 5-HT7 receptors in different motoneuron populations in spinal Onuf＇s nucleus of male rats,and their relationship with the central modulation of the urogenital functions. Methods：Ten adult SD rats（eight male rats and two female rats）were randomly divided into gender control group（n=4, include two male and two female rats）and retrograde tracing group（male rats,n=6）. In gender control group,after the spinal L5-6 segments were removed and cut,sections were treated with 5-HT2A or 5-HT7 receptors immunohistochemical stain. In retrograde tracing group,recombinant pseudorabies virns（PRV-152 ）were injected into right ischiocavernosus muscles（IC group;n=3）or right external urethral sphincter（EUS group;n=3）,respectively. Four days after injection,the L2A segments were removed,cut,and treated with 5-HT2A or 5-HT7 receptors double-labeling immunofluorescence stain. Sections were observed by light microscopy or the laser confocal microscopy to detect the distribution of 5-HT2A,5-HT7 receptors in Onuf＇s nucleus. Results：5-HT2AA receptors immunoreactivity mainly located in the medial part of the dorsolateral nucleus（DLN）of Onuf＇s nucleus,and 5-HT7 receptors mainly located in the lateral part of the DLN;5-HT2A and 5-HT7 receptors expression of male rats in Onuf＇s nucleus were more dense than female rats. Immunofluorescence double-labeling detection exhibited that 5-HT2A receptors mainly distributed in surrounding motoneurons innervating the IC muscles,and 5-HT7 receptors mainly expressed in neurons innervating the EUS. Conclusion： 5-HT2A and 5-HT7 receptors are heterogeneously distributed in different motoneurons of Onuf＇s nucleus innervating different striated muscles. 5-HT2A receptor might be preferentially involved in regulation of the sexual reflex,and the 5-HT7 receptor may be mainly participated in the micturition reflex.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第8期918-922,共5页
Journal of Chongqing Medical University
基金
重庆市自然科学基金(CSTC2006BB5037)
