摘要
目的探讨荧光原位杂交技术(FISH)在膀胱癌诊断中的价值,并与常规的尿脱落细胞学对比。方法收集100例疑似尿路移行上皮细胞肿瘤的血尿患者在行膀胱镜检查之前的尿液标本,分别做尿细胞学检测和荧光原位杂交分析。20例正常人尿液标本,用于建立FISH阈值,作为阳性判断的标准。结果细胞学和FISH的总体灵敏度分别为43.2%、82.4%,特异度分别为:92.3%、88.5%,诊断符合率分别为:56.0%、84.0%,细胞学和FISH在灵敏度、诊断符合率、非浸润性肿瘤和初发肿瘤等方面的差异均有统计学意义。结论在膀胱癌诊断中FISH较常规细胞学具有较高的灵敏度和诊断符合率,更易检出早期肿瘤。
Objective To assess the value of fluorescence in situ hybridization （FISH） in the diagnosis of bladder cancer. Methods Urine samples from 100 patients suspected of having bladder cancer were collected before cystoscopy for immediate urine cytology and FISH analysis. The criteria for FISH abnormality were determined by evaluating the urine specimens from 20 subjects without urogenital neoplasm. Results The overall sensitivity of cytology and FISH was 43.2% and 82.4%, and their specificity was 92.3% and 88.5%, with diagnostic concordance rate of 56.0% and 84.0%, respectively. The differences between FISH and cytology showed statistical significance in the sensitivity, diagnostic concordance rate, non-muscle-invasive cancer and primary cancer. Conclusion The sensitivity and efficiency of FISH in the detection of bladder cancer are superior to those of cytology, especially for prophase cancer.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2010年第7期1597-1599,1603,共4页
Journal of Southern Medical University
基金
卫生部科研基金(WKJ2007-3-001)
关键词
荧光原位杂交
膀胱肿瘤
细胞学
fluorescence in situ hybridization
bladder cancer
cytology
