摘要
目的了解巢式聚合酶链反应（ＮＰＣＲ）技术对支气管内膜结核的诊断价值。方法应用巢式聚合酶链反应（ＮＰＣＲ）对６７份支气管内膜活检组织进行结核分支杆菌ＤＮＡ检测，并与病理检查、刷检涂片、支气管镜检后痰涂片和痰培养结果比较。对照为４３例支气管肺癌患者。结果６７例支气管内膜结核活检组织病理检查、刷检涂片、支气管镜检“激惹”后痰涂片、镜检术后痰培养及ＮＰＣＲ检测阳性率分别为１３％、１９％、２２％、１５％和７６％，后者与前四者相比差异有非常显著性（Ｐ均＜０．０１）。４３例支气管肺癌患者结核分支杆菌ＤＮＡ的ＮＰＣＲ无一例阳性。结论巢式ＰＣＲ技术有益于支气管内膜结核患者的诊断，特别是对胸片正常、痰菌阴性。
Objective To evaluate the nested polymerase chain reaction (NPCR) technique in diagnosing endobronchial tuberculosis.Methods NPCR method was used to detect Mycobacterium tuberculosis DNA in bioptic tissue from 67 patients with endobronchial tuberculosis, and the results were compared with pathologic examination, brushing smear, sputum smear and culture for Mycobacterium tuberculosis after bronchoscopy. The controls were 43 patients with lung cancer.Results The positive rates of detecting Mycobacterium tuberculosis were13%,19%,22%,15% and 76% respectively by pathologic examination, brushing smear, sputum smear, culture after bronchoscopy and NPCR methods. Significant differences were found between NPCR and the other 4 methods (all Ps<0.01). No positive result for Mycobacterium tuberculosis was found in 43 controls by NPCR. Conclusions NPCR is a useful method for diagnosing patients with endobronchial tuberculosis, especially for those with normal demonstration in X ray chest film, negetive sputum and nonspecific pathologic changes in endobronchial biopsy.
出处
《中华结核和呼吸杂志》
CSCD
北大核心
1999年第3期145-146,共2页
Chinese Journal of Tuberculosis and Respiratory Diseases
