摘要
目的:对天花粉中总三萜皂苷含量进行测定,为天花粉质量评价提供依据。方法:选用熊果酸为对照品,5%香草醛-冰醋酸和高氯酸为显色剂,以紫外分光光度法测定天花粉中总三萜皂苷的含量。结果:标品在16～48μg范围内有良好的线性关系(r=0.9905)。样品在10～40min内稳定,加样回收率为97.22%,RSD=1.59。结论:该方法操作简便,快速,灵敏度高和重复性好,可用于天花粉及其相关产品的质量控制。
Objective： To determine the quantity of total triterpenoids in radix trichosanthin. Methods： Total triterpenoids was determined by spectrometry using ursolic acid as atandard and 5% vanillin - glacial acetic and perchloric acid as colcr - developing agents. Results： The content of the standard was closely correlated to its optical density in the range of 16～48 μg （ r = 0.990 5） with a stability within 10 -40 min. The average recoveries was 97.22%, RSD = 1.59. Conclusion： With simple operation, rapidity, high sensitivity as well as fine repetition, this method can be helpful for monitoring the quality of radix trichosanthin and related products.
出处
《中国野生植物资源》
2010年第4期61-63,共3页
Chinese Wild Plant Resources
基金
河南科技学院博士基金(2007009)
关键词
天花粉
总三萜皂苷
分光光度法
radix trichosanthin
total triterpenoids
spectrometry
