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亚硫酸氢盐预处理对玉米秸秆酶水解的影响 被引量:6

Effect of Bisulfite Pretreatment on Enzymatic Hydrolysis of Corn Stalk
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摘要 研究了亚硫酸氢钠对玉米秸秆进行预处理的条件,主要考察了亚硫酸氢钠用量、预处理温度和pH值对玉米秸秆酶水解效率的影响。结果表明:在温度180℃、保温30min时,随着亚硫酸氢钠用量的增加,木质素和半纤维素的溶出量增大,从而促进玉米秸秆预处理后底物的酶水解,当亚硫酸氢钠用量为7%时,酶水解转化率和葡萄糖得率分别为69.40%和62.44%;预处理温度越高,酶水解效率越高,温度190℃、保温30min时底物酶水解转化率达到了81.04%,葡萄糖得率71.91%;预处理pH值升高,酶水解效率相应增大,pH值在4.2~4.7之间时,酶解效率增加明显。 The effects of bisulfite pretreatment conditions including pretreatment temperature, the dosage of sodium bisulfite and pH value on the efficiency of enzymatic hydrolysis of corn stalk were studied. The results showed that the dissolution quantity of enzymatic hydrolysis and glucose yield increased with sodium bisulfite increasing when temperature was 180℃ and holding time was 30min. The enzymatic hydrolysis of the substrate increased while dissolution of both lignin and hemicellulose increased, the conversion of enzymatic hydrolysis was 69.40% and the yield of glucose was 62.44% when bisulfite dosage was 7%. Efficiency of enzymatic hydrolysis increased with higher pretreatment temperature. Conversion of enzymatic hydrolysis of the substrate was achieved 81.04% and the glucose yield reached 71.91% when the temperature was 190℃, retention time was 30min, and sodium bisulfite dosage was 7%. pH Value had great effect on the efficiency of enzymatic hydrolysis. When the pH value was in the range of 4.2-4.7, the efficiency of enzymatic hydrolysis rose significantly.
出处 《林产化学与工业》 EI CAS CSCD 北大核心 2010年第4期73-77,共5页 Chemistry and Industry of Forest Products
基金 天津市高等学校科技发展基金计划项目(20080522)
关键词 玉米秸秆 预处理 酶水解 亚硫酸氢钠 corn stalk pretreatment enzymatic hydrolysis sodium bisulfite
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