摘要
在取材部位、培养条件、培养方式以及在培养基中添加抗氧化剂等方面,对防止黄连木茎段褐化进行了研究。结果表明:半木质化的枝条为适合的外植体;培养初期以液体培养基较好;添加1.4 g/L抗坏血酸(Vc)的抗褐化效果最佳,其次为聚乙烯吡咯烷酮(PVP)和硝酸银(AgNO3),而硫代硫酸钠(Na2S2O3)和活性炭(AC)没有达到预期的效果。对外植体进行适当时间的低温和黑暗处理可减轻褐变。
Different parts of stems,culture conditions and methods,and antioxidant were studied to prevent the browning in Pistacia chinensis Bunge stems tissue culture.The results showed that： semi-lignified branches were the most appropriate.Liquid medium was better at initial stage.The best antibrowning effect could be got when adding 1.4g/L Vc into medium,followed by adding PVP and AgNO3,while adding Na2S2O3 and AC into medium had no expective effects.Furthermore,the explants treaded by proper dark and low temperature could grow better with little browning.
出处
《河南农业科学》
CSCD
北大核心
2010年第8期110-113,共4页
Journal of Henan Agricultural Sciences
基金
国家自然科学青年基金项目(30700078)
河南科技大学博士启动基金(09001062)
河南科技大学SRTP资助(2009130)
关键词
黄连木
茎段
组织培养
褐化
Pistacia chinensis Bunge
Stem
Tissue culture
Browning
