摘要
目的研究糙海参(Holothuria scabraJaeger)具有生物活性的三萜皂苷类成分。方法应用大孔树脂柱色谱、正相硅胶柱色谱、反相硅胶柱色谱和HPLC对糙海参体内三萜皂苷活性成分进行分离纯化,根据化合物的理化性质和波谱数据鉴定其结构。结果分离并鉴定了5个三萜皂苷类化合物,分别为:scabraside C(1)、holothurin A1(2)、echinoside A(3)、24-dehydroechioside A(4)和holothurin A(5),化合物1～5对人白血病细胞HL-60、MOLT-4和人肺癌细胞A-549的抑制率较强。结论化合物1～5为首次从糙海参中分离得到,其中化合物1为新化合物。5个化合物均显示显著的体外细胞毒活性。
Aim To study the bioactive triterpene glycosides of sea cucumber Holothuria scabra Jaeger.Methods Triterpene glycosides in the sea cucumber were isolated and purified by column chromatography on DA-101,silica gel,reversed-phase silica and reversed HPLC.Their chemical structure were identified on the basis of chemical evidence and spectral data.The cytotoxicities of the compounds obtained were tested with HL-60 cells and MOLT-4 cancer cells.Results Five triterpene glycosides were obtained from the sea cucumber studied and their structures were identified as scabraside C（1）,holothurin A1（2）,echinoside A（3）,24-dehydroechinoside A（4） and holothurin A（5）.Their inhibition activities on HL-60,MOLT-4 and A-549 cells were significant.Conclusion Compounds 15 are obstained from Holothuria scabra Jaeger for the first time.All glycosides good show antitumor activity in vitro.
出处
《中国药物化学杂志》
CAS
CSCD
2010年第4期290-297,共8页
Chinese Journal of Medicinal Chemistry
基金
国家高技术研究发展计划项目(863项目
2006AA09Z417)
国家自然科学基金项目(20772155)
关键词
糙海参
三萜皂苷
细胞毒活性
Holothuria scabra Jaeger
triterpene glycosides
cytotoxity
