摘要
目的：研究槲皮素对ＴＮＦα诱导的内皮细胞与中性粒细胞粘附的抑制作用及作用机制。方法：用髓过氧化酶法测定中性粒细胞与内皮细胞的粘附，ＥＬＩＳＡ法测定内皮细胞粘附分子的表达。结果：ＴＮＦα通过增加／诱导内皮细胞ＩＣＡＭ１，ＶＣＡＭ１及Ｅｓｅｌｅｃｔｉｎ的表达而剂量、时间依赖性地增加内皮细胞与中性粒细胞的粘附。槲皮素可剂量依赖性地抑制上述粘附，其作用机制为抑制ＴＮＦα诱导内皮细胞上述３种粘附分子的表达。结论：槲皮素通过抑制ＩＣＡＭ１，ＶＣＡＭ１及Ｅｓｅｌｅｃｔｉｎ的表达而降低ＴＮＦα诱导的内皮细胞与中性粒细胞的粘附。
AIM: To study the effect of quercetin on TNF α induced endothelial neutrophil adhesion and its mechanism. METHODS: Cell adhesion was measured by testing neutrophil myeloperoxidase activity; expression of adhesion molecules was measured by ELISA. RESULTS: The adhesion rate of unactivated human umbilical endothelial cells (EC) to neutrophils was very low. TNF α(100～1000 kU·L -1 ) increased the adhesion of EC to neutrophils both dose and time dependently. Quercetin(1～500 μmol·L -1 ) dose dependently inhibited the adhesion of TNF α stimulated EC to neutrophils. The expression of ICAM 1 and VCAM 1 on the surface of resting EC was very low and no E selectin expression was detectable. TNF α increased the expression of these adhesion molecules and resulted in upregulation of endothelial neutrophil adhesion. CONCLUSION: Quercetin inhibited TNF α stimulated endothelial neutrophil adhesion by suppressing the expressions of ICAM 1, VCAM 1 and E selectin.
出处
《药学学报》
CAS
CSCD
北大核心
1999年第7期491-494,共4页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
