摘要
目的观察不脱钙同种异体骨与脱钙骨基质(DBM)移植后的生长特点,为临床选择填充材料及术后处理提供依据。方法选用新西兰大耳兔右侧尺骨制作骨缺损模型,分别将不脱钙同种异体骨与DBM植入骨缺损处,另作不植入任何物质的骨缺损作空白对照。于2、4、8、12周时摄χ线片观察,于4、8、12周时行大体观察、组织形态学观察及荧光双标观测。结果在实验的各个时间段,不脱钙同种异体骨组的成骨均不如DBM组活跃。不脱钙同种异体骨组总的骨质逐渐减少,8-12周时骨质才重新增多;DBM组骨质持续增多,12周时骨修复基本完成。结论不脱钙同种异体骨与DBM移植后具有不同的生长特点,可以利用这些特点选择骨缺损填充材料以及术后的进一步处理方式。
Objective To study the growth characteristics of non-decalcified bones and demineralized bone matrix （DBM） after transplantation, and to provide the basis for choosing filling materials and postoperative treatment. Methods We established a segmental bone-defect model on the right ear of Zelanian rabbits, and then implanted non-decalcified bones and DBM respectively in these models, and kept untreated rabbits as the control group. X-ray was performed 2, 4, 8 and 12 weeks after the transplantation. Gross observation, Goldner’s histological test and fluorescent labeling were done 4, 8, and 12 weeks after the transplantation. Results The non-decalcified bones showed lower activity of bone regeneration than the DBM at all time points. The bone content of non-decalcified bones gradually reduced, and regenerated 8 weeks after the transplantation. The bone content of DBM gradually increased until the bone regeneration was completed 12 weeks after the transplantation. Conclusions Non-decalcified bones and DBM after transplantation show different growth characteristics which are the basis for choosing substitute materials and postoperative treatment in the clinic.
出处
《中国骨肿瘤骨病》
2010年第4期355-359,363,共6页
Chinse Journal Of Bone Tumor And Bone Disease
