摘要
目的:研制一种新型的、可注射的磷酸钙骨水泥,并测试该骨水泥的成分、理化学特性及其在松质骨内的生物降解和成骨特性,探讨该骨水泥在松质骨内的代谢情况.方法:根据相关文献合成一种含有β-磷酸三钙、磷酸二氢钙、碳酸钙和纳米相羟基磷灰石的磷酸钙骨水泥,并用含藻酸钠的液相成分将该骨水泥调制成可用注射器和16号穿刺针操作的注射物.在新西兰大白兔的股骨髁部钻取直径为2mm的骨洞,注入该骨水泥.在术后4、8、12、16周分别取标本,骨脱钙后石蜡包埋并行HE染色,光镜下观察该骨水泥在松质骨内的降解及新骨再生情况.扫描电镜观察骨-骨水泥界面的超微结构.结果:采用Gillmore双针法测试该型骨水泥的初凝时间为25～30min,终凝时间为17～20h,扫描电镜发现该骨水泥的颗粒较小、孔隙率较高.术后切片见骨水泥在松质骨内相容性良好.4周时骨水泥在体内开始降解,12周为降解反应的高峰期,16周降解反应基本完成,降解同时有大量的骨小梁生成.结论:该型磷酸钙骨水泥可操作性强、生物相容性好、具有骨传导和骨诱导作用,有望成为老年骨质疏松性椎体压缩骨折治疗中较好的填充剂.
Objective:To develop a new and injectable calcium phosphate cement(CPC) and to investigate its components,physicochemical and biological characters in cancellous bone.Method:The cement was mainly composed of β-tricalcium phosphate(β-TCP), dicalcium phosphate anhydrous(DCPA),calcium carbonate and hydroxyapatite(HA) in nano-grade.Sodium alginate solution was added in to the cement in order to make it could be injected through a 16-gauge needle.The cement was injected into cylindrical defects(2mm in diameter) in the femoral condyles of New Zealand rabbits.And specimens were harvested in 4 to 16 weeks after operation,which were then decalcified,embedded in paraffin and cut into 10μm sections,stained by hematoxylin and eosin for histopathologic examinations.At the same time,the specimens was cut and observed by scanning electron microscope(SEM) for ultrastructures.Result:The initial and final setting time was 25~30 minutes,17~20 hours respectively by Gillmore needles at 37℃ and 100% moisture.Small particles and large lacunae in the interface were seen by scanning electron microscope(SEM). The histological study indicated that the cement had excellent compatibility in cancellous bone,which degraded from 4 weeks,reached climax in 12 weeks and terminated in 16 weeks after operation.At the same time,much more new trabeculae bone were formed when the cement disappeared.Conclusion:The new cement has the characters such as injectable,biocomptable,osteoconductive and osteotransductive,which might be a candidate material for use as a bone substitute in the treatment of osteoporosis and vertebral compression fractures(VCFs).
出处
《中国脊柱脊髓杂志》
CAS
CSCD
2005年第1期38-41,i001,共5页
Chinese Journal of Spine and Spinal Cord
基金
广东省科技计划项目资助课题(项目编号20034369)
关键词
可注射性磷酸钙骨水泥
松质骨
骨替代
动物实验
Injectable calcium phosphate cement(ICPC)
Cancellous bone
Bone substitute
Animal study
