摘要
为了研究SRY基因和Y染色体上微卫星标记对性别鉴定的影响,试验以哺乳动物为研究对象,采用多重PCR技术扩增绵羊和牛基因组中Y染色体上的2个微卫星标记和SRY基因,根据其基因型进行性别鉴定,试图通过一次DNA扩增同时提供性别鉴定和基因分型的信息。结果表明:所设计的SRY基因引物具有高度特异性,是性别鉴定的主要依据;Y染色体上的MCM158、MAF45两标记由于特异性不好而不适用于性别鉴定。
To study the effect of the SRY gent and mierosatellite markers on Y chromosome on the sex identification, mammal were used as the research object in this experiment, The technique of multiple PCR was used to amplify two microsatellite markers on Y chromosome and SRY gene to identify sex by genotyping, tried to provide simultaneously information of the sex - identification and genotyping in one step. The result showed that the SRY primers were highly specific and could be used as the main basis for sex - identification. But primer specificity was not good for Y chromosome markers MCM158 and MAF45, and therefore these markers were not suited for sex identification.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2010年第8期4-6,共3页
Heilongjiang Animal Science And veterinary Medicine
基金
德国联邦科技与教育部(BMBF)资助项目(CHN00316)
