摘要
目的:建立岩白菜药材中有效成分熊果苷、岩白菜素、儿茶素HPLC含量测定方法,为其药材质量评价和资源合理利用提供科学依据。方法:采用反相C18色谱柱,流动相为甲醇-水,流速为1.0 mL.m in-1,检测波长为275 nm,对岩白菜属植物岩白菜和厚叶岩白菜的主根、须根和叶片等不同部位中的熊果苷、岩白菜素、儿茶素含量进行HPLC分析比较。结果:岩白菜和厚叶岩白菜中的熊果苷、岩白菜素、儿茶素含量存在一定差异;同植物不同部位中的熊果苷、岩白菜素、儿茶素含量存在明显差异。主根和须根中岩白菜素的含量较高,而叶中熊果苷的含量较高。结论:本研究方法可以同时测定熊果苷、岩白菜素和儿茶素的含量,为岩白菜属植物的科学研究及药材质量评价奠定基础。
Objective： To develop a HPLC method for the determination of arbutin,bergenin and catechin in Chinese herb Bergenia,and to provide a scientific basis for evaluating the quality and reasonable utilization of the herb.Method： The HPLC analysis was achieved by using a C18 column and methanol-water as the mobile phase,with a flow rate of 1.0 mL min-1,and detected by UV at 275 nm.The contents of arbutin,bergenin and catechin in the different parts of axial root,fibrous root and blade from Bergenia purpurascens and B.crassifolia.Result： The contents of arbutin,bergenin and catechin have a few difference in B.purpurascens and B.crassifolia,and varies significantly in the different part of axial root,fibrous root and blade from some species.The contents of bergenin are higher in axial root,fibrous root,and the content of arbutin is higher in blade.Conclusion： This HPLC method can be used to determine simultaneously the content of arbutin,bergenin and catechin,and can establish a foundation for scientific study and evaluating the quality of species in Bergenia.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第16期2079-2082,共4页
China Journal of Chinese Materia Medica
基金
<中国药典>2010版一部标准研究(YS-219)
