摘要
目的：建立王不留行中王不留行黄酮苷的含量测定方法。方法：运用高效液相色谱法,Alltima-C18（4.6 mm×250mm,5μm）色谱柱,以甲醇-0.3%磷酸溶液为流动相,梯度洗脱,流速1.0 mL.min-1,检测波长280 nm。结果：王不留行黄酮苷在0.102-1.539μg线性关系良好（R2=0.999 7）,平均加样回收率为100.4%,RSD 0.81%。10批样品含量测定表明,王不留行黄酮苷质量分数为0.46%-0.57%。结论：本法简便、准确、重复性好、专属性强,为王不留行药材质量控制提供了方法。
To establish a HPLC method for the determination of vaccarin in Vaccariae Semen.Analysis was carried out on an Alltima-C18 column（4.6 mm×250 mm,5 μm） eluted with methanol-0.3% phosphoric acid as mobile phase in gradient elution.The flow rate was 1.0 mL·min-1 and detected wavelength was set at 280 nm.The peak areas and injection ammounts of vaccarin showed a good linear relationship in the range of 0.102-1.539 μg,R2=0.999 7.The average recovery was 100.4%,RSD was 0.81%.The results of the assay of 10 samples showed that the contents of vaccarin varied in the range of 0.46%-0.57%.The method is simple,accurate,reproducible and specific.It can be used for the quality control of Vaccariae Semen.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第16期2072-2074,共3页
China Journal of Chinese Materia Medica
基金
<中国药典>2010版一部标准研究(YD-158)
