摘要
目的：建立中药苏木的质量标准。方法：采用薄层色谱法,以巴西苏木素和（±）原苏木素B为对照,建立其定性鉴别;采用醇溶性浸出物测定法,建立浸出物的测定;采用HPLC建立其主要有效成分巴西苏木素和（±）原苏木素B的含量测定。结果：采用硅胶GF254薄层板,以三氯甲烷-丙酮-甲酸（8∶4∶1）为展开剂,苏木中各成分能够得到较好分离,收集的18批样品显示基本相同的斑点。18批样品中醇溶性浸出物的质量分数为6.4%~11.3%。含量测定的方法学考察表明,巴西苏木素在0.362~5.425μg,（±）原苏木素B在0.313~4.695μg,各自峰面积与进样量呈良好的线性关系,回归方程分别为Y=798 999.57X-219 666.54（r=0.999 7）,Y=930 296.63X-123 330.67（r=0.999 5）;平均回收率分别为98.6%,100.5%。18批样品含量测定表明,巴西苏木素的质量分数为1.18~17.26 mg.g-1,（±）原苏木素B的质量分数为5.94~17.04 mg.g-1,不同样品的含量有明显的差别。结论：建立的薄层色谱鉴别方法具有很强的专属性,可用于苏木的鉴别。建立的HPLC含量测定方法简便、重现性好、准确度高,可用于苏木药材的质量控制。
To establish the quality standard of Sappan Lignum,TLC and HPLC method were employed.The components of Sappan Lignum could be separated well on GF254 thin tayer plate using a mixture of chloroform-acetone-formic acid（8∶4∶1） as the mobile phase,and the 18 samples collected basically showed the same spots.The ethanol-soluble extractives of 18 samples varied in the range of 6.4% to 11.3%.The methodological investigation of assay showed,the peak areas and the injection ammount of brazilin and（±）protosappanin B were in good linear correlation when their amounts were in the ranges of 0.362-5.425 μg and 0.313-4.695 μg,with the regression equations of Y=798 999.57X-219 666.54（r=0.999 7） and Y=930 296.63X-123 330.67（r=0.999 5） and the average recoveries were 98.6% and 100.5%,respectively.The contents differed significantly among the samples.The TLC identification method established was suitable to identify Sappan Lignum due to its strong specificity.The HPLC assay method established could be applied to the quality control of Sappan Lignum due to its convenience,good reproducibility and high accuracy.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第16期2068-2071,共4页
China Journal of Chinese Materia Medica
基金
<中国药典>2010版一部标准研究项目(YS-037)
